Gatan sc1000 orius ccd camera

PEG-AuNPs and BIOT-NFL-PEG-AuNPs were observed by transmission electron microscopy (TEM) at the Service Commund’Imageries et d'Analyses Microscopiques (SCIAM; University of Angers, France). 2 μL of each sample was deposited on copper grids (150 mesh) and stained with 2% uranyl acetate for one minute, and then each sample was dried at room temperature before observation. The examination was performed using a 120 kV Jeol JEM-1400 electron microscope (Jeol, Japan) equipped with a Gatan SC1000 ORIUS^® CCD camera (11 Megapixel) from the USA.

C. albicans strain wild type strain UC820 (Table 1) was cultured overnight in YEPD + uri broth at 30 °C with shaking at 200 rpm. The culture was diluted in fresh YEPD + uri to an OD₆₀₀ of 0.2 and incubated for 4 h at 30 °C with shaking at 200 rpm. Cells were frozen under high pressure, freeze substituted and embedded in Spurr’s resin as described in Section 2.3. Thick sections (200 nm) were transferred onto 200 mesh copper grips and stained with uranyl acetate and lead citrate. A single axis tilt series of a region of the C. albicans cell wall was captured between ± 65° at 1° intervals using a JEM1400 TEM (Jeol Ltd.) set at 120 kV and equipped with a bottom mounted Gatan SC1000 ORIUS CCD camera (Gatan).
The TEM images were compiled into a single axis tomogram using the eTomo interface of IMOD v4.7 image processing package freely available from http://bio3d.colorado.edu/imod/ (Kremer et al., 1996 (link)). The full tomogram, reconstructed using the back-projection method, comprised of 2672 × 190 × 2672 slices along the x, y and z axes, respectively. This was further trimmed and realigned to eliminate out of focus slices from the edge of the tilt series. The final tomogram used for modelling comprised of 1545 × 693 × 77 slices, with a voxel resolution of 0.272 nm³.