Tertiapin q

The following chemicals were products of R&D Systems: NT, TTX, kynurenic acid, picrotoxin, SR 48692, GDP-β-S, U73122, U73343, heparin, thapsigargin, chelerythrine, bisindolylmaleimide II (Bis II), ML 133, and tertiapin-Q (TQ). Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP₂) was purchased from Echelon Biosciences. PD149163 was product of Millipore Sigma. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on NT-elicited facilitation of AP firing (data not shown).

The following chemicals were purchased from R&D Systems: NMB, kynurenic acid, picrotoxin, U73122, U73343, heparin, thapsigargin, BAPTA, chelerythrine, bisindolylmaleimide II (Bis II), phorbol 12-myristate 13-acetate (PMA), TTX, ML 133, tertiapin-Q (TQ), tertiapin-LQ, BIM23042, and PD168368. Stock solutions of drugs were prepared and aliquoted for storage at −20°C until use. For chemicals dissolved in dimethyl sulfoxide (DMSO), the final concentration of DMSO was less than 0.1%.

The following chemicals were products of R&D Systems: AVP, TTX, kynurenic acid, picrotoxin, 6,7-dinitroquinoxaline-2,3-dione (DNQX), SR49059, GDP-β-S, U73122, U73343, heparin, thapsigargin, chelerythrine, bisindolylmaleimide II (Bis II), ML 133, ML 297, tertiapin-Q and tertiapin-LQ. Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP₂) was purchased from Echelon Biosciences. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.