Rabbit anti-EGFR antibodies (D38B1) and phosphor-EGF receptor Tyr1068 (2236) were purchased from Cell Signaling Technology (Danvers, MA, USA). The mouse anti-β-actin antibody (A5441) was purchased from Sigma-Aldrich (Oakville, ON, Canada). The mouse anti-GFP antibody (JL-8) was obtained from Clontech (Palo Alto, CA, USA). Anti-EGFR mAb (cetuximab C225) was purchased from MedChemExpress (Shanghai, China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies were purchased from Jackson Immuno-research Inc. (West Grove, PA, USA). The EGFR-GFP transfection plasmid (32,751) was purchased from Addgene (Watertown, MA, USA), and a DNA ligation kit (6022) was obtained from Takara Bio Inc. (Shiga, Japan).
Mouse anti β actin antibody a5441
Manufactured by Merck Group
Sourced in United States
The Mouse anti-β-actin antibody (A5441) is a laboratory reagent used to detect the presence of the β-actin protein in biological samples. β-actin is a widely expressed cytoskeletal protein that is commonly used as a reference or control in various cellular and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti ha antibody 16b12, by BioLegend (2 mentions)
Mouse anti gfp antibody jl 8, by Takara Bio (1 mentions)
Horseradish peroxidase hrp conjugated goat anti mouse and anti rabbit antibodies, by Jackson ImmunoResearch (1 mentions)
Las 4000, by Fujifilm (1 mentions)
Immobilon p membrane, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Nacalai Tesque (1 mentions)
Chemi lumi one super, by Nacalai Tesque (1 mentions)
Blocking one, by Nacalai Tesque (1 mentions)
Ab51074, by Abcam (1 mentions)
Bca protein assay reagent, by Beyotime (1 mentions)
Fd8030, by Fdbio (1 mentions)
Rat anti lamp1 1d4b, by Santa Cruz Biotechnology (1 mentions)
6 protocols using mouse anti β actin antibody a5441
1
EGFR Signaling Pathway Antibody Analysis
2
Western Blot Analysis of EPHB2 Protein
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Cells were lysed in RIPA buffer containing protease inhibitor cocktail (Nacalai Tesque). The protein concentration of lysates was measured using Bio-Rad DC kits (Bio-Rad Laboratories, Inc.). Cell lysates (20 µg of protein) were separated by 4–12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and then electroblotted onto Immobilon-P membranes (EMD Millipore, Billerica, MA, USA). Membranes were blocked with Blocking-one (Nacalai Tesque) overnight at 4°C, and incubated with goat polyclonal anti-EPHB2 antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or with mouse anti-β-actin antibody (A5441; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 4°C. After 24 h of incubation, membranes were washed with PBS containing 0.1% Tween-20 (PBS-T), followed by incubation with horseradish peroxidase-conjugated secondary antibody for rabbit or mouse (GE Healthcare Life Science, Little Chalfont, UK), for 1 h at room temperature. The membranes were washed extensively with PBS-T, and treated with Chemi-Lumi-One Super (Nacalai Tesque) to visualize immunoreactive signals using LAS4000 (Fujifilm, Tokyo, Japan).
3
Antibody Sources for Cellular Signaling
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The CAIX antibodies (goat anti-mouse CAIX AF2344, goat anti-human CAIX AF2188, monoclonal mouse anti-human CAIX MAB2188) and the mouse anti-IgG2A (MAB003) isotype control were obtained from R&D systems (Minneapolis, MN, USA). The mouse monoclonal anti-cortactin (ab33333), function-blocking mouse monoclonal anti-MMP14 (ab78738) and rabbit polyclonal anti-MMP14 (ab51074) antibodies were obtained from Abcam (Cambridge, MA, USA). The rabbit anti-Tks5 (aka FISH; sc-30122), goat anti-integrin β1 (M-106) (sc-6622), mouse anti-integrin β1 (P5D2) (sc-13590), goat anti-α2 integrin (N-19) (sc-6586), goat anti-CD98 (C-20) (sc-7095), mouse anti-CD98 (F-2) (sc-390154), and rabbit anti-FAK (A-17) (sc-557) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The mouse anti-β1-integrin (Cat no. 556048) antibody was obtained from BD Biosciences (Mississauga, Ontario, Canada). The mouse anti-α2-integrin antibody (clone-P1E6, Cat no. MAB 1950) was obtained from Millipore (Etobicoke, ON, Canada). The rabbit anti-cofilin (D3F9 XP®) (5175) antibody was obtained from Cell Signalling Technology Inc (Danvers, MA, USA). The mouse anti-β-actin antibody (A5441) was obtained from Sigma-Aldrich (St Louis, MO, USA).
4
Western Blot Analysis of PGC1β, CPT1α, and CPT1β
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Total protein was isolated from the liver in a lysis buffer for 30 min at 4°C. Protein concentration was measured using BCA Protein Assay Reagent (P0011, Beyotime Biotechnology, China). The proteins were transferred to a polyvinylidene difluoride membrane, blocked with 5% nonfat dry milk in PBS with 0.02% (v/v) Tween-20, and incubated with primary antibodies at 4°C overnight. The membrane was washed and incubated for 1 h at room temperature with a peroxidase-labeled secondary antibody. After washing, protein bands were visualized by electrochemiluminescence (FD8030, FDBio Science, China). Anti-Pgc1β (A17089), Cpt1α (A5307), and Cpt1β (A6796) were obtained from ABclonal (Wuhan, China). Mouse anti-β-actin antibody (A5441) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Antibody Purchasing Protocol for Western Blot
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A rabbit anti-AQP5 antibody (AQP005) was purchased from Alomone Labs (Jerusalem, Israel). A mouse anti-HA antibody (16B12) was purchased from Biolegend (San Diego, CA, USA). A mouse anti-β-actin antibody (A5441) and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG (A9044) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HRP-conjugated donkey anti-rabbit IgG (711-035-152) was purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11008) and Alexa Fluor 647-conjugated anti-mouse IgG (A21235) were purchased from Invitrogen (Carlsbad, CA, USA).
6
Antibody Acquisition for Cellular Imaging
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Rabbit anti-HA antibody (600-401-384) was purchased from Rockland Immunochemicals (Gilbertsville, PA, USA). Mouse anti-HA antibody (16B12) was purchased from Biolegend (San Diego, CA, USA). Mouse anti-calnexin (AF18), EEA1 (E-8), TGN38 (B-6), and rat anti-LAMP1 (1D4B) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-p62 antibody (PM045) was purchased from MBL (Nagoya, Japan). Mouse anti-β-actin antibody (A5441) and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG antibody (A9044) were purchased from Sigma Aldrich (St. Louis, MO, USA). HRP-conjugated donkey anti-rabbit IgG antibody (711-035-152) was purchased from Jackson ImmunoReseach Laboratories (West Grove, PA, USA). Alexa Fluor 488 (A11008)- and 647 (A21244)-conjugated goat anti-rabbit IgG antibody and Alexa Fluor 647-conjugated anti-mouse IgG antibody (A21235) were purchased from Invitrogen (Carlsbad, CA, USA).
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