Mab672

Chemotaxis assay was performed using 24-well Transwell plates (Costar, 3415, USA). One milliliter supernatants from ME180 cells which were mock-infected or infected with HSV-2, or mock-transfected or transfected with ICP4 expressing plasmid were added to the lower chamber. To examine the roles of CXCR3 and CXCR3 ligands in mediating cell migration, supernatants or cells were incubated with anti-CXCL9 (10 μg/mL, R&D Systems, MAB392, USA), -CXCL10 (2 μg/mL, R&D Systems, MAB266, USA), -CXCL11 (2 μg/mL, R&D Systems, MAB672, USA) or -CXCR3 (1 μg/mL, R&D Systems, MAB160, USA) neutralizing Abs, respectively, for 1 h, according to the manufacturer's instructions. Activated PBMCs and CD4⁺ T cells (5 × 10⁵) in 100 μL RPMI-1640 medium were added to the upper chamber. The chambers were incubated for 2 h at 37°C in a 5% CO2 incubator. Cell migrated to the lower chambers were collected and counted using an automatic cell counter (Bio-Rad).

MHCC-97H and Huh-7 cells were cultured with NF-CM, CAF-CM or CAF-CM containing different concentrations of CXCL11 recombinant protein (10876-HNAE, SinoBiological, China) or CXCL11 neutralizing antibody (MAB672, R&D Systems, USA) for 72 h. Then, Transwell, wound healing, IF, and western blot assays were performed.