Wb4101

Manufactured by Bio-Techne

Sourced in United Kingdom

The WB4101 is a high-performance western blotting system from Bio-Techne. It is designed to provide efficient and reliable protein transfer and detection in western blotting applications.

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5 protocols using wb4101

In Vivo Optical Imaging of Neurotransmitter Responses

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Mice were prepared and injected with CNiFERs as described. Additionally, a 0.1 MΩ tungsten bipolar stimulating electrodes with a tip separation of 500 μm (Microprobes Inc.) was implanted into either substantia nigra (−3.2 mm A/P, −1.3 mm M/L, −4.4 mm D/V) or locus coeruleus (−5.3 mm A/P, −0.9 mm M/L, −3.4 mm D/V). After a day of recovery, imaging was performed under isoflurane anesthesia. Experimental runs consisted of 30s baseline followed by electrical stimulation (200 μs pulses of 50 – 300 μA at 50 Hz for 500 ms). To test the specificity of the response, eticlopride (1mg/kg, Sigma), WB4101 (2 mg/kg, Tocris), or cocaine (15mg/kg; Sigma) were injected i.p. 10 minutes before the electrical stimulation.

Muller A., Joseph V., Slesinger P.A, & Kleinfeld D. (2014). Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex. Nature methods, 11(12), 1245-1252.

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In Vivo Optical Imaging of Neurotransmitter Responses

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Muller A., Joseph V., Slesinger P.A, & Kleinfeld D. (2014). Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex. Nature methods, 11(12), 1245-1252.

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Adrenoceptor Antagonist Cocktail Protocol

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WB4101 (selective α₁-adrenoceptor antagonist; Tocris, Westwoods Business Park, Ellisville, MO, USA; cat. # 0946), RX821002 (selective α₂-adrenoceptor antagonist; Tocris, cat. # 1324), propranolol (β-adrenoceptor antagonist; Sigma–Aldrich, St. Louis, Missouri, USA; cat. # P0884), tribromoethanol (Sigma–Aldrich, cat. # T48402), and urethane (Sigma–Aldrich; cat. # U2500) were dissolved in saline (NaCl 0.9%). Flunixin meglumine (Banamine, Schering Plough, Cotia, São Paulo, Brazil) and the polyantibiotic preparation of streptomycins and penicillins (Pentabiotico, Fort Dodge, Campinas, São Paulo, Brazil) were used as provided.

Oliveira L.A., Pollo T.R., Rosa E.A., Duarte J.O., Xavier C.H, & Crestani C.C. (2021). Both Prelimbic and Infralimbic Noradrenergic Neurotransmissions Modulate Cardiovascular Responses to Restraint Stress in Rats. Frontiers in Physiology, 12, 700540.

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Pharmacological Agents for Neuroscience Research

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ARC-239, BRL-44408, clonidine hydrochloride, dexmedetomidine hydrochloride, efaroxan hydrochloride, guanabenz acetate, guanfacine hydrochloride, idazoxan hydrochloride, JP-1302, prazosin hydrochloride, rauwolscine hydrochloride, RS-79948, RX-821002, terazosin, UK-14304, WB-4101 and yohimbine hydrochloride were acquired from Tocris (Ellisville, MO). Atipamezole was from Orion Corporation (Espoo, Finland) and isoflurane was from Abbott Diagnostics (Chicago, IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).

Biggane J.P., Xu K., Goldenstein B.L., Davis K.L., Luger E.J., Davis B.A., Jurgens C.W., Perez D.M., Porter J.E, & Doze V.A. (2022). Pharmacological characterization of the α2A-adrenergic receptor inhibiting rat hippocampal CA3 epileptiform activity: Comparison of ligand efficacy and potency. Journal of receptor and signal transduction research, 42(6), 580-587.

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Antagonizing α1-Adrenergic Receptors During Recordings

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To antagonize the α1-adrenergic receptors extracellularly during both extracellular and intracellular recordings (Figures 2G and 7), borosilicate pipettes used for LFP recordings (see above) were filled with WB4101 (2-(2,6-Dimethoxyphenoxyethyl)aminomethyl-1,4-benzodioxane hydrochloride; Tocris Bioscience, UK) dissolved in 0.9% NaCl to a concentration of 30 μM.¹⁶ (link) In order to avoid leak of inhibitor during LFP baseline pre-recording, gentle negative pressure was applied during the pre-drug application phase. Then gentle positive pressure was applied with a pressure-injection device (NIM module, Heidelberg, Germany) until 0.5 mL of WB4101 solution or vehicle was infused over 5 min. The injection was the same for both extra- and intra-cellular recordings. Recordings were then repeated 30 min after drug or vehicle control injection (excluding perfusion time).

Lorenzon P., Antos K., Tripathi A., Vedin V., Berghard A, & Medini P. (2023). In vivo spontaneous activity and coital-evoked inhibition of mouse accessory olfactory bulb output neurons. iScience, 26(9), 107545.

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