2 mercaptoethanol

Manufactured by Solarbio

Sourced in China

2-mercaptoethanol is a clear, colorless liquid chemical compound. It is commonly used as a reducing agent in various biochemical applications, including protein purification, cell lysis, and gel electrophoresis.

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3 protocols using 2 mercaptoethanol

Cell Culture of NK-92MI Cells

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NK-92MI cells were obtained from Procell Life Science&Technology Co, Ltd. They were cultures in αMEM(Gibco, Cat#41061029) medium supported with 12.5% heat-inactivated FBS(Hyclone, Cat#SH30088.03HI), 12.5% heat-inactivated horse serum (Gibco, Cat#26050088), 0.2 mM inositol (Solarbio, Cat#I8050), 0.1 mM 2-mercaptoethanol (Solarbio, Cat#M8210), and 0.02 mM folic acids (Solarbio, Cat#P1400). All media were supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin (Solarbio, Cat#P1400). Cells were cultivated at 37 °C in a humidified atmosphere of 5% CO₂ in the air.

Lin L., Liu X., Yu H., Deng H., Peng K., Chen J., Zhang C., Jiang T, & Liu X. (2023). Inhibitory effect and related mechanism of decitabine combined with gemcitabine on proliferation of NK/T cell lymphoma cells. Frontiers in Pharmacology, 14, 1134895.

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Mung Bean Protein Extraction and Characterization

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Peeled mung bean was obtained from Zhanchuang Agricultural Technology Co. Ltd., (Shandong, China). Alcalase (176,743 U/g) was acquired from Novo Co. Ltd., (Beijing, China). 1-anilino-8-naphthalene sulfonate (ANS) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were from Sigma Co., Ltd. (St. Louis, MO USA). Tris-HCl buffer, sodium dodecyl sulphate (SDS), glycerol, bromophenol blue, 2-mercaptoethanol, Coomassie brilliant blue (R-250), trichloroacetic acid (TCA), potassium bromide, pyrogallol, salicylic acid, ferrous sulfate, potassium ferricyanide, ferric chloride, and ferrous chloride were from Beijing Solarbio Technology Co. Ltd. (Beijing, China). All chemicals used in this study were of analytical grade.

Liu F.F., Li Y.Q., Sun G.J., Wang C.Y., Liang Y., Zhao X.Z., He J.X, & Mo H.Z. (2022). Influence of ultrasound treatment on the physicochemical and antioxidant properties of mung bean protein hydrolysate. Ultrasonics Sonochemistry, 84, 105964.

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Culturing NKTCL and NK-92 MI Cell Lines

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Three NKTCL cell lines—SNT-8, SNK-10, and SNT-16 (kind gifts from Dr. Norio Shimizu at Tokyo Medical and Dental University)—were cultured in RPMI-1640 medium (Hyclone) supplemented with 10% human plasma (Gemini) and 700 U/mL recombinant human interleukin-2 (PeproTech). The NK-92 MI cell line was purchased from ATCC (CRL-2408) and cultured in Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides (Gibco) but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate (Life Technologies) supplemented with 12.5% fetal bovine serum (Biological Industries), 12.5% horse serum (Biological Industries), 0.2 mM inositol (Solarbio), 0.1 mM 2-mercaptoethanol (Solarbio) and 0.02 mM folic acid (Sigma Aldrich). All cells were cultivated at 37°C in a humidified atmosphere of 5% CO₂ in the air.

Zhang C., Xu H., Sui X., Chen L., Chen B., Lv H., Wang S, & Wang X. (2022). Icaritin inhibits PLK1 to activate DNA damage response in NK/T cell lymphoma and increases sensitivity to GELOX regime. Molecular Therapy Oncolytics, 25, 288-304.

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