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Sodium dodecyl sulfonate sds

Manufactured by Merck Group
Sourced in United States

Sodium dodecyl sulfonate (SDS) is a laboratory reagent used as a detergent and surfactant. It functions as an anionic surfactant, reducing the surface tension of aqueous solutions.

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3 protocols using sodium dodecyl sulfonate sds

1

SDS-PAGE Protein Analysis Protocol

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Spectroscopy grade KBr (Tianjin Jinbeier Tech. Co., Ltd., Tianjin, China), SDS-PAGE standard protein (Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai, China), acrylamide (Sangong Biotech, Shanghai, China), methylene bisacrylamide (Sigma, St. Louis, MO, USA), trishydroxymethylaminomethane (Tris, Sigma), sodium dodecyl sulfonate (SDS, Sigma), ammonium persulfate (Sigma), Coomassie Brilliant Blue R-250 (Sigma), acetic acid (No. 3 Chemical Reagent Factory, Tianjin, China), methanol (Tian Da Reagent Factory, Tianjin, China), tetramethylethylenediamine (TEMED, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), horseradish peroxidase (HRP, Guo Yuan Biotechnology Co., Ltd., Shanghai, China), glucono-δ-lactone (Nanjing Chemical Reagent Co., Ltd., Nanjing, China). Unless stated otherwise all reagents were analytical purity quality.
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2

Synthesis of Acrylic Copolymer Emulsion

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Reagent grade methylmethacrylate (MMA), butyl acrylate (BA), acrylic acid (AA) and hydroxyl propyl acrylate (HPA) as monomers, ammonium peroxodisulfate (APS) as an initiator, OP-10 as a non-ionic emulsifier, sodium bicarbonate (NaHCO3) as a buffer and propylene glycol methyl ether acetate (PGMEA) as a remover were obtained from Aladdin Reagent Co. Ltd. (Shanghai, China). Sodium dodecyl sulfonate (SDS) as an anionic emulsifier was purchased from Sigma-Aldrich Corporation (St. Louis, Mo USA). An aqueous solution of 25% ammonia as a neutralizer was supplied by Tianjin Fuyu Fine Chemical Co., Ltd. (Tianjin, China). All reagents were used as received, without further purification.
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3

Hydrocarbon-utilizing Bacteria Growth Media

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Nutrient broth medium (NB) contained (g/l): 15 peptone, 3 yeast extract, 1 glucose and 6 NaCl. The basal medium (BM) consisted of 0.3 g KH2PO4, 0.4 g NH4Cl, 0.33 g MgCl2.6H2O, 0.05 CaCl2.2H2O g, 30 g NaCl and 1 ml trace-element solution [12] (link) per liter of distilled water. The culture media were sterilized by autoclaving at 121 °C for 20 min.
Complex hydrocarbons including diesel fuel and motor oil were obtained from Shell Company (Sfax, Tunisia) and sterilized by filtration (pore size 0.45 µm; Millipore), while crude oil was collected from "Thyna Petroleum Services" (Sfax, Tunisia) and sterilized by autoclaving. Olive oil and corn oil were obtained from commercial sources. Residual frying oil was collected from a restaurant located in Sfax, Tunisia. These vegetable oils were sterilized by filtration (pore size 0.45 µm; Millipore). The solutions of glycerol and chemical surfactants including Tween 20, Tween 80, Titon X-100 and sodium dodecyl sulfonate (SDS) were purchased from Sigma Aldrich Company (98-99 % purity). Chemical surfactants were dissolved in water at concentrations corresponded at their CMCs and sterilized by filtration (pore size 0.45 µm; Millipore).
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