Hyaluronic acid 250 kda

Soybean oil and surfactant Lipoid E80 (egg lecithin powder 80–85% enriched with phosphatidylcholine and 7–9.5% content in phosphatidylethanolamine) were purchased from Lipoid GmbH and used without further purification. For preparation of all nanoemulsions and solutions, Millipore Milli-Q water was used. Chitosan (CT, LMW 90–150 kDa, DDA 84% determined via 1H-NMR). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), biotin hydrazide, curcumin (from Curcuma longa Turmeric, powder), streptavidin (SAV), 1-hydroxybenzotriazole hydrate (HOBt), N,N′-diisopropylcarbodiimide (DIC), N,N-diisopropylethylamine (DIEA), trifluoroacetic acid (TFA), dimethyl sulfoxide (DMSO), dichloromethane (DCM), anhydrous N,N-dimethyl-formamide (DMF), N-methyl-2-pyrrolidone (NMP), 1,2-ethanedithiol (EDT), triisopropylsilane (TIS) piperidine, acetone, diethyl ether, dimethyl sulfoxide (DMSO) and deuterium oxide (D₂O) were purchased from Sigma Aldrich. Hyaluronic acid 250 kDa and biotin–PEG_2k–maleimide were purchased from Creative PEGWorks. Atto 655-SAV was purchased from Atto-Tec. Dialysis membranes were purchased from Spectrum Laboratories Inc. Hyaluronic acid 250 kDa and biotin–PEG_2k–maleimide were purchased from Creative PEGWorks. N-α-Fmoc amino acids, HCTU and 5-carboxy fluorescein were purchased from NovaBiochem.

Materials: Both soybean oil (density at 20°C of 0.922 g mL⁻¹) and surfactant Lipoid E80 (egg lecithin powder 80–85% enriched with phosphatidylcholine (PC) and 7–9.5% content in phosphatidylethanolamine (PE)) were purchased from Lipoid GmbH and used without further purification. Millipore Milli-Q water was used for the preparation of all nanoemulsions and solutions. Chitosan (CT, LMW 90–150 kDa, DDA 84% determined via ¹H-NMR) was purchased from Sigma Aldrich (Milan, Italy). Hyaluronic acid 250 kDa were purchased from Creative PEG Works (HA) and used without further purification. Ubidecarenone, CoenzymeQ₁₀(CoQ₁₀) was kindly offered from Faravelli. Human Fetal Cardiomyocytes (HFC cells) and human liver cells (THLE-2 cell line) (CRL-2706™) were purchased from American Type Culture Collection, Manassas, VA, USA.