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Loricrin

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Loricrin is a protein expressed in the granular layer of stratified squamous epithelia, including the epidermis. It is a major structural component of the cornified cell envelope, which provides mechanical strength and barrier function to the skin.

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Lab products found in correlation

Filaggrin, by Santa Cruz Biotechnology (2 mentions) Cytokeratin14, by GeneTex (2 mentions) Cytokeratin15, by GeneTex (2 mentions) Cytokeratin10, by GeneTex (2 mentions) Fluorescently coupled secondary anti rabbit or rat igg, by Cell Signaling Technology (2 mentions) Streptavidin conjugated cy5, by BioLegend (2 mentions) Leica dmi6000b fluorescence microscope, by Leica Microsystems (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Involucrin, by Fortrea (2 mentions) Keratin 1, by Abcam (1 mentions) Keratin 5, by Fortrea (1 mentions)

3 protocols using loricrin

1

Immunostaining of Skin Cryosections

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Skin samples were fixed in 4% paraformaldehyde,dehydrated in 30% sucrose, embedded in OCT and frozen. Skin cryostat sections (5mm) were stained with antibodies specific for YFP (life technologies, Grand Island, NY), OX40 (BD Biosciences), filaggrin (Santacruz, Dallas, TX), involucrin (Covance, Princeton, NJ), loricrin, cytokeratin14, cytokeratin15, cytokeratin10 (Genetex, Irvine, CA) Ki67, CD4 (eBioscience), cleaved caspase 3 (Cellsignaling), and TSLP (R&D Systems, Minneapolis, MN), then stained with fluorescently-coupled secondary anti-rabbit or -rat IgG (Cellsignaling Technologies, Danvers, MA), or streptavidin-conjugated Cy5 (Biolegend). Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).
Yu M., Owens D.M., Ghosh S, & Farber D.L. (2015). Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease. The Journal of investigative dermatology, 135(11), 2688-2696.
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2

Immunostaining of Skin Cryosections

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Skin samples were fixed in 4% paraformaldehyde,dehydrated in 30% sucrose, embedded in OCT and frozen. Skin cryostat sections (5mm) were stained with antibodies specific for YFP (life technologies, Grand Island, NY), OX40 (BD Biosciences), filaggrin (Santacruz, Dallas, TX), involucrin (Covance, Princeton, NJ), loricrin, cytokeratin14, cytokeratin15, cytokeratin10 (Genetex, Irvine, CA) Ki67, CD4 (eBioscience), cleaved caspase 3 (Cellsignaling), and TSLP (R&D Systems, Minneapolis, MN), then stained with fluorescently-coupled secondary anti-rabbit or -rat IgG (Cellsignaling Technologies, Danvers, MA), or streptavidin-conjugated Cy5 (Biolegend). Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).
Yu M., Owens D.M., Ghosh S, & Farber D.L. (2015). Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease. The Journal of investigative dermatology, 135(11), 2688-2696.
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3

Immunodetection of Collagen XVII

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Immunodetection was performed with antibodies against murine collagen XVII: EC-7 against the C-terminal ectodomain, Endo-2 against the endodomain (Franzke et al., 2002) ; human collagen XVII: NC16a-1, detects full-length collagen XVII (Hofmann et al., 2009) , HK139 selective for the shed ectodomain (Nishie et al., 2010) , integrin a6 subunits (Progen, Heidelberg, Germany), integrin b4 subunits (BD Pharmingen, Heidelberg, Germany), laminin-332 (a kind gift from Dr. K. Yancey, University of Texas, Dallas), keratin 5 (Covance, Munich, Germany), keratin 1 (Abcam, Cambridge, UK), transglutaminase 1 (Santa Cruz, Heidelberg, Germany), and loricrin (GeneTex, Irvine, CA).
Jacków J., Schlosser A., Sormunen R., Nyström A., Sitaru C., Tasanen K., Bruckner-Tuderman L, & Franzke C.W. (2016). Generation of a Functional Non-Shedding Collagen XVII Mouse Model: Relevance of Collagen XVII Shedding in Wound Healing. The Journal of investigative dermatology, 136(2).
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