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Polyethersulfone filters

Manufactured by GE Healthcare
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Polyethersulfone filters are a type of lab equipment used for filtration in various applications. These filters are designed to effectively remove particulates, macromolecules, and other contaminants from liquid samples. They are known for their durability, chemical resistance, and ability to maintain high flow rates during the filtration process.

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Lab products found in correlation

Protamine sulfate, by Merck Group (2 mentions) Polyfect, by Qiagen (2 mentions)

2 protocols using polyethersulfone filters

1

Lentiviral Gene Transduction in HEK293T Cells

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For lentiviral gene transduction, HEK293T cells transfected with the respective lentiviral vectors and packaging plasmids psPAX2 and pMD2.G using Polyfect (QIAGEN) or PEI. 24h later, medium was exchanged to RPMI, supplemented with 10% (v/v) FBS and antibiotics (100 U/ml penicillin, 100 mg/ml streptomycin). 48h after transfection, cell supernatants were harvested, filtered through 0.45 μm polyethersulfone filters (GE Healthcare) and supplemented with 8 μg/ml protamine sulfate (Sigma). Cells were infected by spinfection (2000 rpm, 45 minutes, room temperature). 24h after infection, medium was changed, 48h after infection, cells were selected with the respective antibiotics.
Heinz L.X., Lee J., Kapoor U., Kartnig F., Sedlyarov V., Papakostas K., César-Razquin A., Essletzbichler P., Goldmann U., Stefanovic A., Bigenzahn J.W., Scorzoni S., Pizzagalli M.D., Bensimon A., Müller A.C., King F.J., Li J., Girardi E., Mbow M.L., Whitehurst C.E., Rebsamen M, & Superti-Furga G. (2020). TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature, 581(7808), 316-322.
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2

Lentiviral Gene Transduction in HEK293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For lentiviral gene transduction, HEK293T cells transfected with the respective lentiviral vectors and packaging plasmids psPAX2 and pMD2.G using Polyfect (QIAGEN) or PEI. 24h later, medium was exchanged to RPMI, supplemented with 10% (v/v) FBS and antibiotics (100 U/ml penicillin, 100 mg/ml streptomycin). 48h after transfection, cell supernatants were harvested, filtered through 0.45 μm polyethersulfone filters (GE Healthcare) and supplemented with 8 μg/ml protamine sulfate (Sigma). Cells were infected by spinfection (2000 rpm, 45 minutes, room temperature). 24h after infection, medium was changed, 48h after infection, cells were selected with the respective antibiotics.
Heinz L.X., Lee J., Kapoor U., Kartnig F., Sedlyarov V., Papakostas K., César-Razquin A., Essletzbichler P., Goldmann U., Stefanovic A., Bigenzahn J.W., Scorzoni S., Pizzagalli M.D., Bensimon A., Müller A.C., King F.J., Li J., Girardi E., Mbow M.L., Whitehurst C.E., Rebsamen M, & Superti-Furga G. (2020). TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature, 581(7808), 316-322.
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