Ai 1000

Manufactured by Vector Laboratories

Sourced in United States

The AI-1000 is a compact automated immunoassay analyzer developed by Vector Laboratories. The device is designed to perform quantitative and qualitative immunoassay tests for various analytes. It utilizes advanced imaging technology and specialized software to automate the analysis process.

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Lab products found in correlation

Axio scan z1 slide scanner, by Zeiss (2 mentions) Ultraview universal dab detection kit, by Roche (1 mentions) Ai 2000, by Vector Laboratories (1 mentions) Eclipse e600 microscope, by Leica (1 mentions) Ab32083, by Abcam (1 mentions)

3 protocols using ai 1000

Immunohistochemical Characterization of Tissue Samples

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Tissues and organoids were fixed in 4% paraformaldehyde, followed by dehydration, paraffin embedding, sectioning, and standard hematoxylin and eosin (H&E) staining and IHC staining. Next, the samples were incubated with primary antibodies, including anti-thyroid transcription factor-1 (TTF-1) (1:200 dilution; Invitrogen Signaling Technology), anti-p40 (1:200 dilution; Cell Signaling Technology), anti-cytokeratin 5 and 6 (1:200 dilution, #MA5-12429; Invitrogen), and anti-programmed death ligand-1 (anti-PD-L1) (1:200 dilution, #13684; Cell Signaling Technology). The sections were subsequently incubated with secondary antibodies (#AI-2000 and #AI-1000; Vector Laboratories, Newark, CA, USA) at 1:5000 dilution and visualized using an ultraView Universal DAB Detection kit (Ventana Medical Systems, Oro Valley, AZ, USA). Nuclei were counterstained with Harris hematoxylin. Images were acquired via a Leica Eclipse E600 microscope [16 (link)].

Park D., Lee D., Kim Y., Park Y., Lee Y.J., Lee J.E., Yeo M.K., Kang M.W., Chong Y., Han S.J., Choi J., Park J.E., Koh Y., Lee J., Park Y., Kim R., Lee J.S., Choi J., Lee S.H., Ku B., Kang D.H, & Chung C. (2023). Cryobiopsy: A Breakthrough Strategy for Clinical Utilization of Lung Cancer Organoids. Cells, 12(14), 1854.

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Immunohistochemical Analysis of PTPRS in RA Synovium

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Mouse monoclonal anti-human PTPRS antibody was obtained from Abnova, and control IgG antibody (unconjugated goat anti-mouse IgG) was purchased from Vector Laboratories (AI-1000). Paraffin-embedded slides of human RA synovial tissue were deparaffinated in xylene, rehydrated, and pretreated for 10 min with boiling citrate antigen retrieval buffer [1.9 mM citric acid and 10 mM tris-sodium citrate (pH 6.0)] and then treated with 3% H₂O₂ for 10 min to quench endogenous peroxidases. Slides were blocked with 5% goat serum for 1 hour at room temperature and then incubated with anti-PTPRS antibody or control rabbit IgG (1:200 in 5% BSA) overnight at 4°C. Slides were washed and incubated with SignalStain Boost IHC (immunohistochemistry) Detection reagent (Cell Signaling Technology) for 30 min, incubated for 2 min with 3,3′-diaminobenzidine substrate, and counterstained with hematoxylin. Slide images were obtained using an Axio Scan.Z1 slide scanner (Carl Zeiss Microscopy).

Svensson M.N., Zoccheddu M., Yang S., Nygaard G., Secchi C., Doody K.M., Slowikowski K., Mizoguchi F., Humby F., Hands R., Santelli E., Sacchetti C., Wakabayashi K., Wu D.J., Barback C., Ai R., Wang W., Sims G.P., Mydel P., Kasama T., Boyle D.L., Galimi F., Vera D., Tremblay M.L., Raychaudhuri S., Brenner M.B., Firestein G.S., Pitzalis C., Ekwall A.K., Stanford S.M, & Bottini N. (2020). Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal. Science Advances, 6(26), eaba4353.

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Immunohistochemical Analysis of SHP-2 in RA Synovium

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The rabbit anti–SHP-2 antibody was purchased from Abcam (ab32083). The control rabbit IgG antibody (unconjugated goat anti-rabbit IgG) was purchased from Vector Labs (AI-1000). Paraffin-embedded slides of human RA synovial tissues were deparaffinated, rehydrated, and pretreated for 10 minutes with boiling citrate antigen retrieval buffer (1.9 mM citric acid, 10 mM Tris-sodium citrate, pH 6.0), and treated with 3% H₂O₂ for 10 minutes. Slides were blocked with 5% goat serum for 1 hour at room temperature and then incubated with anti–SHP-2 antibody or control rabbit IgG (1:200 in 5% BSA) overnight at 4°C. Slides were washed and incubated with SignalStain Boost IHC Detection reagent (HRP, rabbit; Cell Signaling Technologies) for 30 minutes, incubated for 2 minutes with 3,3′-diaminobenzidine substrate, and counterstained with hematoxylin. Slide images were obtained using an Axio Scan.Z1 slide scanner (Carl Zeiss Microscopy).

Maeshima K., Stanford S.M., Hammaker D., Sacchetti C., Zeng L.F., Ai R., Zhang V., Boyle D.L., Aleman Muench G.R., Feng G.S., Whitaker J.W., Zhang Z.Y., Wang W., Bottini N, & Firestein G.S. (2016). Abnormal PTPN11 enhancer methylation promotes rheumatoid arthritis fibroblast-like synoviocyte aggressiveness and joint inflammation. JCI Insight, 1(7), e86580.

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