Dp22 camera

Manufactured by Olympus

Sourced in Japan

The DP22 camera is a digital camera designed for microscope imaging applications. It features a 2.8-megapixel CMOS sensor and can capture high-resolution images. The camera is compatible with a variety of microscope eyepieces and can be connected to a computer for image capture and analysis.

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19 protocols using dp22 camera

Microscopic Imaging Techniques in Research

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Some of the images were acquired with Olympus IX53 inverted microscope equipped with Olympus DP75 camera (resolution of 5760 × 3600 pixels and pixel size of 5.86 × 5.86 µm) with Cell Sens Entry software (version 1.17). Images were acquired with 10X (numerical aperture 0.50) objective lenses at 1600 × 1200 pixels and 72.0 1/in resolution in both X and Y axis. The rest of the images were acquired using Olympus IX73 inverted microscope with Olympus DP22 camera (resolution of 1920 × 1440 pixels and pixel size of 3.69 × 3.69 µm) and DP camera acquisition software. Images were acquired with 4X (numerical aperture 0.13) or at 1920 × 1440 pixels resolution.

Hammoudeh S.M., Hammoudeh A.M, & Hamoudi R. (2019). High-throughput quantification of the effect of DMSO on the viability of lung and breast cancer cells using an easy-to-use spectrophotometric trypan blue-based assay. Histochemistry and Cell Biology, 152(1), 75-84.

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Cultivation and Branching of Conchocelis

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Single-celled conchocelis were statically cultured in wells containing sterilized artificial seawater under the conditions described above except that they were aerated. Swelling tip cells of conchocelis side branches were then detected by microscopy, and their growth and side-branch formation were monitored for 7 days using an Olympus IX73 light microscope equipped with an Olympus DP22 camera. The branching rate was calculated as the number of cells producing a branch as a percentage of the total number of cells observed.

Taya K., Takeuchi S., Takahashi M., Hayashi K.I, & Mikami K. (2022). Auxin Regulates Apical Stem Cell Regeneration and Tip Growth in the Marine Red Alga Neopyropia yezoensis. Cells, 11(17), 2652.

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Histopathological Evaluation of Sternebrae

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Sternebrae collected during the hematopoietic study were fixed in 10% zinc-buffered formalin for at least 24 h and up to 7 days. Fixed sternebrae were decalcified for 3 h in 12–18% sodium EDTA (pH 7.4–7.5) and specimens dehydrated using graded ethanol concentrations and embedded in paraffin. Longitudinal 4 μm sections were stained with regular hematoxylin and eosin. Two board-certified pathologists conducted histopathological evaluation of the samples. One of the pathologists scored all the samples blindly. Bone marrow was evaluated in situ within sternebrae and graded (grade 1: ≤10%; grade 2: 11–30%; grade 3: 31–60%; grade 4: 61–89%; grade 5: ≥90%) for total cellularity. Megakaryocytes were also quantified based on the average per 10 high power fields (HPF) at 400× magnification using a BX43 or BX53 microscope (Olympus, Minneapolis, MN). Images were captured with an Olympus DP22 camera and imported into Olympus cellSens Standard software for review.

Jurgensen K.J., Skinner W.K., Oronsky B., Abrouk N.D., Graff A.E., Landes R.D., Culp W.E., Summers TA J.r, & Cary L.H. (2021). RRx-001 Radioprotection: Enhancement of Survival and Hematopoietic Recovery in Gamma-Irradiated Mice. Frontiers in Pharmacology, 12, 676396.

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Contractility Measurement of Rat Jejunum Muscularis

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Muscularis externa was dissected from the jejunum of 6–8 weeks old Sprague Dawley rat as previously described.11 (link) Tissues were transduced with non-targeting or let-7f lentiviral particles (Dharmacon) with a multiplicity of infection of 5. At day 0 and day 5, about 2 min long movies were recorded at a rate of 14 frames/second using a DP22 camera (Olympus) connected to a SZ61 stereomicroscope (Olympus) and used for contractility measurement and spatial–temporal mapping.

Mazzone A., Strege P.R., Gibbons S.J., Alcaino C., Joshi V., Haak A.J., Tschumperlin D.J., Bernard C.E., Cima R.R., Larson D.W., Chua H.K., Graham R.P., El Refaey M., Mohler P.J., Hayashi Y., Ordog T., Calder S., Du P., Farrugia G, & Beyder A. (2019). microRNA overexpression in slow transit constipation leads to reduced NaV1.5 current and altered smooth muscle contractility. Gut, 69(5), 868-876.

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Skeletal Staining and Imaging Protocol

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Alcian Blue and Alizarin Red stained embryos were brightfield imaged in whole mount in 50% glycerol and 0.2% KOH on an Olympus SZX7 microscope (Shinjuku, Tokyo, Japan) with an Olympus DP22 camera. Flat mounts were prepared by manually separating dorsal neurocranial tissues from ventral viscerocranial tissues via an insect pin, as previously described (Kimmel et al., 1995 (link)). Flat mount images were acquired on a Zeiss AxioImager.A1 compound microscope (Oberkochen Germany) with a Zeiss AxioCam HRc camera. Linear measurements of embryos were determined using ImageJ (NIH).

Everson J.L., Batchu R, & Eberhart J.K. (2020). Multifactorial Genetic and Environmental Hedgehog Pathway Disruption Sensitizes Embryos to Alcohol‐Induced Craniofacial Defects. Alcoholism, Clinical and Experimental Research, 44(10), 1988-1996.

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Anchorage-Independent Growth Assay

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For the estimation of capacity of gene-edited cells for anchorage-independent growth, we used protocol suggested by Du et al. [57 (link)]. A total of 5000 cells per well of a 12-well plate were embedded in 0.5 mL of 0.35% agar, prepared on full MCF10-A medium. A total of 0.5 mL of 0.8% agar, made on full MCF10A medium, was used as a bottom layer. During 3 weeks of cell growth, 0.5 mL of fresh medium was added on the top of soft agar every 4–5 days. Pictures of cell colonies were taken on the Olympus CKX53 microscope supplied with the DP22 camera (Olympus, Tokyo, Japan). Colony size was analyzed using the Fiji software (freehand selection tool). Results are expressed as the means and standard errors of the mean (SEM) from three independent experiments. Statistical analysis was performed in the GraphPad software using ANOVA test.

Dayoub A., Fokin A.I., Lomakina M.E., James J., Plays M., Jacquin T., Novikov N.M., Vorobyov R.S., Schegoleva A.A., Rysenkova K.D., Gaboriaud J., Leonov S.V., Denisov E.V., Gautreau A.M, & Alexandrova A.Y. (2022). Inactivation of PTEN and ZFHX3 in Mammary Epithelial Cells Alters Patterns of Collective Cell Migration. International Journal of Molecular Sciences, 24(1), 313.

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In situ Hybridization and Alizarin Red Staining

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Riboprobes were generated from either plasmids or PCR templates and in situ hybridisation was performed as previously described (Oates and Ho, 2002 (link)). Whole mount stained embryos were documented on an Olympus SZX10 stereoscope with a QImaging Micropublisher camera. Flat-mounted embryos were photographed on an Olympus MVX10 stereoscope with an Olympus DP22 camera. Alizarin Red bone staining was performed as described previously (Spoorendonk et al., 2008 (link)) for fish between 8 weeks and one year of age.

Lleras Forero L., Narayanan R., Huitema L.F., VanBergen M., Apschner A., Peterson-Maduro J., Logister I., Valentin G., Morelli L.G., Oates A.C, & Schulte-Merker S. (2018). Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock. eLife, 7, e33843.

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Microscopic Assessment of Tumor Samples

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Images were taken at 40×, 100× and 200× magnifications using an Olympus BX43 light microscope fitted with an Olympus DP22 camera. Images were collected using DP software and analysed by comparing the intensity of the label in the tumour sample to that of the matched non-tumour tissue.

Venugopal A., Michalczyk A., Khasraw M, & Ackland M.L. (2022). EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms. International Journal of Molecular Sciences, 23(21), 13645.

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Quantitative Analysis of Particulate Shed

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An in vitro bench-top model was used to analyze the particulates shed from n = 3 of the MR-TMD-DCBs. The methods of the model are provided in our previous study.^{8 (link)} The particulates were imaged using an Olympus SZX10 stereo microscope equipped with Olympus DP22 camera and the number of particulates and average size of the particulates were quantitatively analyzed (>100 μm) using ImageJ. For proper visualization, the white particulates were artificially colored green.

Anderson J.A., Lamichhane S., Fuglsby K., Remund T., Pohlson K., Evans R., Engebretson D, & Kelly P. (2019). Development of Drug-Coated Balloon for the Treatment of Multiple Peripheral Arterial Segments. Journal of vascular surgery, 71(5), 1750-1757.e7.

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Alcian Blue and Alizarin Red Staining Protocol

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Alcian Blue‐ and Alizarin Red‐stained embryos were brightfield imaged in whole mount in 50% glycerol and 0.2% KOH on an Olympus SZX7 microscope (Shinjuku, Tokyo, Japan) with an Olympus DP22 camera. Flat mounts were prepared by manually separating dorsal neurocranial tissues from ventral viscerocranial tissues via an insect pin, as previously described (Kimmel et al., 1995). Flat‐mount images were acquired on a Zeiss AxioImager.A1 compound microscope (Oberkochen Germany) with a Zeiss AxioCam HRc camera. Linear measurements of embryos were determined using ImageJ (NIH).

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