Anti gapdh and anti β actin antibodies

Cocaine hydrochloride, FITC-dextran, MMP inhibitor (GM6011), prolidase inhibitor (N-Cbz-L-proline), iNOS inhibitor (S-methylthiourea [SMT]), N- (1-Naphthyl) ethylenediamine dihydrochloride (NEDD), sulfanilamide (SULF) vanadium (III) chloride (VCl3), potassium nitrate, and Prolidase substrate (Gly-Pro) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Prolidase (a gift from Dr. James Phang, NCI/NIH), anti-phospho Ser/Thr and anti-iNOS antibodies were from Cell Signaling Technology (Danvers, MA, USA), and anti-eNOS was procured from the Bethyl Laboratories (Montgomery, TX, USA) and anti-GAPDH and anti-β actin antibodies were procured from Sigma-Aldrich (St. Louis, MO, USA). Human Brain Microvascular Endothelial Cells (HBMECs) were purchased from Sciencell (Carlsbad, CA). THP-1 cell line was obtained from the American Type Culture Collection (ATCC) (Manassas, VA). The TZM-bl reporter cell line was obtained from Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc., through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH.

Cocaine hydrochloride, all-trans-retinoic acid (ATRA), and nomifensine were purchased from Sigma-Aldrich. Anti-PARP-1 and anti-caspase-9 antibodies were from Cell Signaling Technology, anti-DAT and anti-tyrosine hydroxylase (TH) antibodies were purchased from Abcam and anti-GAPDH and anti-β actin antibodies were procured from Sigma-Aldrich.