Catechin (C, ≥98%), galloCatechin (GC, ≥98%), galloCatechin-3-gallate (GCG, ≥98%), epiCatechin (EC, ≥98%), epiCatechin-3-gallate (ECG, ≥98%), epigalloCatechin (EGC, ≥98%), epigalloCatechin-3-gallate (EGCG, ≥98%), caffeine (CAF, ≥98%), theaflavins (TF, ≥95%), theaflavins-3-gallate (TF3G, ≥98%), theaflavin-3′-gallate (TF3′G, ≥98%), theaflavine-3,3′-digallate (TFDG, ≥98%), quercetin-3-O-galactoside (≥98%), myricetin-3-O-galactoside (≥98%), kaempferol-3-O-rutinoside (≥98%), vitexin (≥98%), astragaloside (≥98%), quercetin (≥98%), and kaempferol (≥98%) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). l -alanine, l -arginine, l -aspartic acid, l -cystine, l -glutamic acid, glycine, l -histidine, l -isoleucine, l -leucine, l -lysine, l -methionine, l -phenylalanine, l -proline, l -serine, l -threonine, l -tyrosine, l -valine, l -asparagine, l -glutamine, theanine, γ-aminobutyric acid, and l -tryptophan were purchased from Sigma (Sigma-Aldrich Co., St. Louis, MO, USA). Anthrone reagent and ninhydrin/formic acid reducing agent were purchased from BeiJing DingGuochangSheng Biotech. Co., Ltd. (Bejing, China). Ethyl caprate (≥99%) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), and n-alkane mixed standard C7-C40 was purchased from o2si (o2si smart solutions—an LGC Standards Company, Charleston, SC, USA).
Gallocatechin
Manufactured by Yuanye Bio-Technology
Sourced in China
Gallocatechin is a chemical compound that belongs to the group of flavan-3-ols, a type of flavonoid. It is a naturally occurring antioxidant found in certain plants.
Automatically generated - may contain errors
Lab products found in correlation
Epicatechin, by Yuanye Bio-Technology (11 mentions)
Epigallocatechin, by Yuanye Bio-Technology (11 mentions)
Catechin, by Yuanye Bio-Technology (9 mentions)
Epicatechin gallate, by Yuanye Bio-Technology (9 mentions)
Epigallocatechin gallate (egcg), by Yuanye Bio-Technology (9 mentions)
Gallocatechin gallate, by Yuanye Bio-Technology (8 mentions)
Gallic acid, by Yuanye Bio-Technology (7 mentions)
Rutin, by Yuanye Bio-Technology (6 mentions)
Kaempferol, by Yuanye Bio-Technology (4 mentions)
Quercetin, by Yuanye Bio-Technology (4 mentions)
Catechin gallate, by Yuanye Bio-Technology (4 mentions)
Formic acid, by Thermo Fisher Scientific (4 mentions)
Myricetin 3 o galactoside, by Yuanye Bio-Technology (3 mentions)
Caffeine, by Yuanye Bio-Technology (3 mentions)
Ellagic acid, by Yuanye Bio-Technology (3 mentions)
Isoquercitrin, by Yuanye Bio-Technology (3 mentions)
Caffeic acid, by Yuanye Bio-Technology (3 mentions)
P coumaric acid, by Yuanye Bio-Technology (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
14 protocols using gallocatechin
1
Analytical Standards for Tea Bioactives
2
Quantification of Flavonoids and Catechins
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Xanthine oxidase powder was purchased from Yuanye Biotechnology Co. (Shanghai, China). Flavone (99.0%), 7-OH flavone (98.0%), chrysin (98.0%), biochanin A (98.0%), naringin (98.0%), and myricetin (98.0%) were bought from Alfa Aesar (Thermo Fisher Scientific, MA, USA). Formononetin (99.0%) and genistein (99.0%) were acquired from Acros Organics (Fisher Scientific, PA, USA). Baicalin (98.0%), baicalein (98.0%), wogonin (98.0%), apigenin (98.0%), luteolin (98.0%), hispidulin (98.0%), tangeretin (98.0%), nobiletin (98.0%), galangin (98.0%), kaempferide (98.0%), kaempferol (98.0%), kaempferitrin (98.0%), quercetin (98.0%), fisetin (98.0%), rutin (98.0%), daidzein (98.0%), tectorigenin (98.0%), puerarin (98.0%), dihydromyricetin (98.0%), naringenin (98.0%), catechin (C; 98.0%), (−)-gallocatechin (GC; 98.0%), epicatechin, (EC; 98.0%), (−)-epicatechin gallate (ECG; 98.0%), (−)-epigallocatechin (EGC; 98.0%), (−)-epigallocatechin gallate (EGCG; 98.0%), and (−)-gallocatechin gallate (GCG; 98.0%) were commercially purchased from Yuanye Biotechnology Co. (Shanghai, China). High-performance liquid chromatography (HPLC) grade acetonitrile was purchased from Merck KGaA (Darmstadt, Germany). Ultrapure water (18.2 MΩ cm resistivity) was obtained from an ELGA water purification system (ELGA Berkefeld, Veolia, Germany). All other reagents and solvents were of analytical grade.
3
Antioxidant Compounds Characterization
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The following standards with purity higher than 98% were purchased from Yuanye Biotechnology Co., Ltd.: isoquercitrin, isorhamnetin‐3‐O‐neohesperidoside, narcissin, quercitrin, ellagic acid, gallocatechin, apigenin, rutin, and kaempferol. Epicatechin was obtained from Chengdu Pulse Biotechnology Co., Ltd. Citric acid, sucrose, tannic acid, sodium glutamate, and quinine hydrochloride was acquired from Adamas Beta Chemical Reagents Co., Ltd. 2,2′‐Azino‐bis‐(3‐ethylbenzthiazoline‐6‐sulphonate acid; ABTS) and ferric ion‐reducing antioxidant power (FRAP) kits were provided by Shanghai Biyuntian Biotechnology Co., Ltd. Diphenyl‐1‐picrylhydrazyls (DPPH) was supplied by Sigma‐Aldrich. Chromatography‐grade formic acid was obtained from Shanghai Aladdin Reagent Co., Ltd. Methanol was acquired from Tianjin Beilian Fine Chemical Depot. Experimental ultrapure water was produced with a Mili‐Q system (Millipore Corp.).
4
Preparation of Polyphenol Standard Solutions
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Chlorogenic acid (CAS: 327-97-9), neoChlorogenic acid (CAS: 906-33-2), trans-cinnamic acid (CAS: 140-10-3), ferulic acid (CAS: 1135-24-6), p-coumaric acid (CAS: 501-98-4), caffeic acid (CAS: 331-39-5), p-hydroxybenzoic acid (CAS: 99-96-7), epicatechin (CAS: 490-46-0), (+)-catechin (CAS: 154-23-4), (−)-epigallocatechin (CAS: 970-74–1), (−)-gallocatechin (CAS: 3371-27-5), phloretin (CAS: 60-82-2) and phlorizin (CAS: 60-81-1) were purchased from Shanghai Yuanye Bio-technology Co., Ltd (Shanghai, China). All phenolic chemicals were analytical standards, with HPLC purity ≥ 98%.
Stock solutions of polyphenols were obtained by dissolving each polyphenol in methanol containing 5% dimethyl sulfoxide (DMSO). Subsequently, each polyphenol was diluted with sterilized NB medium to the appropriate concentration. All solutions were prepared fresh and filter-sterilized (pore size, 0.22 μm) prior to use.
Stock solutions of polyphenols were obtained by dissolving each polyphenol in methanol containing 5% dimethyl sulfoxide (DMSO). Subsequently, each polyphenol was diluted with sterilized NB medium to the appropriate concentration. All solutions were prepared fresh and filter-sterilized (pore size, 0.22 μm) prior to use.
5
Standardization of Tea Compound Analysis
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The references of non-volatile compounds (HPLC grade ≥ 98%) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China), including catechins (catechin, epicatechin, catechin gallate, epicatechin gallate, gallocatechin, epigallocatechin, gallocatechin gallate and epigallocatechin-3-gallate), alkaloids (caffeine, theobromine and theophylline), flavonol glycosides (quercetin-3-O-glucoside, quercetin-3-O-galactoside, quercetin-3-O-rutinoside, quercetin-3-O-rhamnosyl-glucoside, myricetin-3-O-glucoside, myricetin-3-O-galactoside, myricetin-3-O-rhamnoside, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-rhamnosyl-glucoside) and free amino acids (glutamic acid, aspartic acid, asparaginate, serine, glutamine, histidine, tryptophan, glycine, phenylalanine, arginine, theanine, alanine, γ-aminobutyric acid, tyrosine, threonine, valine, isoleucine, leucine and lysine). Acetonitrile and methanol were HPLC grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The ultra-pure water (>18 MΩ cm) was prepared by Milli-QTM reference system (Merck Millipore, Milford, MA, USA).
6
Polyphenol Quantification and Characterization
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The (+)-catechin, (−)-epicatechin, (−)-epigallocatechin, and (−)-epicatechin gallate (+)-gallocatechin and (−)-epigallocatechin gallate, (purity > 98 %) were purchased from Shanghai Yuanye Biotechnology Co. ltd (Shanghai, China). Methanol, acetonitrile, acetic acid, formic acid, hydrochloric acid (HPLC grade), gallic acid, rutin, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Shanghai, China). Phloroglucinol and ascorbic acid were purchased from Shandong Xiya Chemistry Industry ltd (Linyi, China). Sodium dodecyl sulfate (SDS), triethanolamine (TEA), and Folin-phenol were purchased from Beijing Solarbio Science & Technology Co., ltd (Beijing, China). Sodium carbonate, sodium hydroxide, aluminum chloride, sodium nitrite, and sodium acetate were purchased from Tianjin Bodi Chemical Co., ltd (Tianjin, China). The C-18 SPE column was obtained from Tianjin Chengzhou Technology Co. ltd (Tianjin, China). The deionized water was obtained from a Milli-Q system (Merck Millipore, Darmstadt, Germany).
7
Comprehensive Analysis of Polyphenol Compounds
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Methanol (HPLC grade, 99.9%), ethanol (HPLC grade, 99.8%), acetonitrile (LC/MS grade, 99.9%), and formic acid (LC/MS grade) were all purchased from Thermo Fisher Scientific Technology Co., Ltd. (Shanghai, China). Standard compounds, namely, punicalagin, punicalin, corilagin, ellagic acid, gallic acid, catechin, epicatechin, epicatechin gallate, gallocatechin, epigallocatechin, epigallocatechin gallate, kaempferol-3-O-glucoside, isoquercitrin, luteolin-7-O-glucoside, naringenin-7-O-glucoside, and rutin, were all purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China).
8
Qingzhuan Tea's Bioactive Compounds
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Tea samples (Qingzhuan tea) were obtained from Huanghelou Tea Factory (Hubei, China); α-glucosidase (EC 3.2.1.20 Saccharomyces cerevisiae, G0660) and p-nitrophenyl-α-D-glucopyranoside (pNPG, N1377) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Catechins standards of (−)-epicatechin (EC), (+)-catechin (C), (−)-epigallocatechin (EGC), (−)-gallocatechin (GC), (−)-epicatechin gallate (ECG), (−)-catechin gallate (CG), (−)-epigallocatechin gallate (EGCG) and (−)-gallocatechin gallate (GCG) and gallic acid (GA) were purchased from Shanghai Yuanye Biological Technology Company Limited (Shanghai, China). Acarbose was acquired from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). All other reagents and solvents were purchased from China National Pharmaceutical Group Corporation (Beijing, China) and were of analytical grade. Purified water (18.2 MΩ) was prepared using a Millipore Mill-Q Ultrapure Water System (Billerica, MA, USA).
9
Profiling Tea Polyphenols and Amino Acids
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(‐)‐Catechin (C), (‐)‐epigallocatechin (EGC), (‐)‐gallocatechin (GC), (‐)‐epicatechin (EC), (‐)‐epicatechin gallate (ECG), (‐)‐epigallocatechin gallate (EGCG), (‐)‐gallocatechin gallate (GCG), anhydrous glucose, caffeine (CAF), and gallic acid (GA) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Company (Shanghai, China). Alanine (ala), arginine (arg), aspartic acid (asp), cysteine (cys), glutamic acid (glu), glycine (gly), histidine (his), isoleucine (ile), leucine (leu), methionine (met), phenylalanine (phe), proline (pro), serine (ser), threonine (thr), tryptophan (trp), tyrosine (tyr), valine (val), theanine, the AccQ‐Fluor Reagent Kit, and AccQ‐Tag eluent were purchased from BDH Chemicals Ltd. (Poole, UK). Acetonitrile (chromatographically pure), sodium carbonate, methanol (chromatographically pure), concentrated sulfuric acid, anthrone, ferrous tartrate (analytically pure), disodium hydrogen phosphate, and potassium dihydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Pure water from China Resources C'estbon Beverage Co., Ltd. (Shenzhen, China) was used throughout the study.
10
Comprehensive Tea Phytochemical Analysis
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Black tea, green tea and jasmine tea were from Eastsign Foods (Quzhou) Co., Ltd. (Quzhou, China). Purified water was from Hangzhou Wahaha Beverage Co., Ltd. (Hangzhou, Zhejiang, China), which had a total dissolved solids of 1.768 mg/L and a pH of 6.74.
Gallic acid (GA), gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), gallocatechin gallate (GCG), epigallocatechin gallate (EGCG), catechin gallate (CG), epicatechin gallate (ECG), theaflavin (TF), theaflavin-3-gallate (TF-3-G), theaflavin-3-gallate (TF-3-G) and theaflavin-3,3-digallate (TFDG) were from Shanghai Yuanye Biotechnology Co., Ltd. (China) with purity ≥95%. N-Alkanes for linear retention index (RI) determination and internal standard solution (decanoic acid ethyl ester) were from TCI (Shanghai) Development Co., Ltd. (Shanghai, China). Ninhydrin, stannous chloride, sodium carbonate, disodium hydrogen phosphate, potassium dihydrogen phosphate and disodium EDTA were all analytical grade from local suppliers. Acetic acid and acetonitrile were chromatographic grade, from local suppliers.
Gallic acid (GA), gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), gallocatechin gallate (GCG), epigallocatechin gallate (EGCG), catechin gallate (CG), epicatechin gallate (ECG), theaflavin (TF), theaflavin-3-gallate (TF-3-G), theaflavin-3-gallate (TF-3-G) and theaflavin-3,3-digallate (TFDG) were from Shanghai Yuanye Biotechnology Co., Ltd. (China) with purity ≥95%. N-Alkanes for linear retention index (RI) determination and internal standard solution (decanoic acid ethyl ester) were from TCI (Shanghai) Development Co., Ltd. (Shanghai, China). Ninhydrin, stannous chloride, sodium carbonate, disodium hydrogen phosphate, potassium dihydrogen phosphate and disodium EDTA were all analytical grade from local suppliers. Acetic acid and acetonitrile were chromatographic grade, from local suppliers.
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