The antibodies used for ChIP were: H3 (Abcam ab1791, lot no. GR177884-2), H3K27ac (Abcam ab4729, lot no. GR200563-1), H3K27me3 (Millipore 07–499, lot no. 2475696), H3K36me3 (Abcam ab9050, lot no. GR204353-1), H3K4me3 (Abcam ab8580, lot no. GR190202-1), and H3K9me3 (Abcam ab8898, lot no. GR186864-1), and were validated by the company for specificity. For the H3K4me3 antibody, abcam reports strong binding to H3K4me3 but some cross reactivity with H3K4me2 [103 ]. The following primary antibodies were used for immunofluorescence: mouse anti-Nc82 (1∶20; Developmental Studies Hybridoma Bank) and rat anti-FruM (1:200) [18 (link)]. The secondary antibodies used for immunofluorescence were Alexa Fluor goat anti-rat 488 (1:1000), goat anti-mouse 633 (1:500), rabbit anti-GFP 488 conjugate (1:600), and streptavidin 488 conjugate (1:4000) (Thermo Fisher).
Rabbit anti gfp 488 conjugate
Manufactured by Thermo Fisher Scientific
Rabbit anti-GFP 488 conjugate is a fluorescent-labeled antibody that binds specifically to green fluorescent protein (GFP). It is designed for use in various immunological and cell biology applications where the detection of GFP-tagged proteins is required.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin 488 conjugate, by Thermo Fisher Scientific (2 mentions)
H3k36me3, by Abcam (2 mentions)
H3k4me3, by Abcam (2 mentions)
H3k9me3, by Abcam (2 mentions)
Mouse anti nc82, by Developmental Studies Hybridoma Bank (2 mentions)
Alexa fluor 488 goat anti rat, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse 633, by Thermo Fisher Scientific (2 mentions)
H3k27me3, by Merck Group (2 mentions)
H3k27ac, by Abcam (2 mentions)
Ab8898, by Abcam (1 mentions)
Ab9050, by Abcam (1 mentions)
Ab8580, by Abcam (1 mentions)
Ab4729, by Abcam (1 mentions)
Ab1791, by Abcam (1 mentions)
2 protocols using rabbit anti gfp 488 conjugate
1
ChIP-seq Antibody Validation and Immunofluorescence
2
Chromatin Immunoprecipitation and Immunofluorescence Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The antibodies used for ChIP were: H3 (Abcam ab1791), H3K27ac (Abcam ab4729), H3K27me3 (Millipore 07-499), H3K36me3 (Abcam ab9050), H3K4me3 (Abcam ab8580), and H3K9me3 (Abcam ab8898), and were validated by the company for specificity. The following primary antibodies were used for immunofluorescence: mouse anti-Nc82 (1∶20; Developmental Studies Hybridoma Bank) and rat anti-Fru M (1:200) (18) . The secondary antibodies used for immunofluorescence were Alexa Fluor goat anti-rat 488 (1:1000), goat anti-mouse 633 (1:500), rabbit anti-GFP 488 conjugate (1:600), and streptavidin 488 conjugate (1:4000) (Thermo Fisher).
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