Rabbit anti adiponectin antibody

Buffers and chemicals used to perform electrophoresis, immunoblotting and RT-qPCR were obtained from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The following antibodies were used for the Western blotting analysis: rabbit anti-C/EBPα (#2295S) and anti-PPARγ antibodies (#2443S) from Cell Signaling Technology (Leiden, The Netherlands); rabbit anti-adiponectin antibody (#GTX112777) from GeneTex Inc. (Ivrine, CA, USA); hFAB rhodamine anti-tubulin (#12004166), hFAB rhodamine anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #12004168) and goat anti-rabbit IgG StarBright Blue 700 (#12004162) antibodies from Bio-Rad. All other materials and analytical-grade substances were purchased from Merck KGaA (Darmstadt, Germany) unless otherwise specified.

Total protein lysates were prepared and analyzed through Western blotting as previously described (Vasileva et al., 2021 (link)). Equivalent amounts of 30 μg of protein samples were resolved on a sodium dodecyl sulfate polyacrylamide gel and transferred to nitrocellulose blotting membranes. The protein data was normalized over a housekeeping protein on Image Lab 6.0.1 software (Bio-Rad). Antibodies were purchased as follows: rabbit anti-AKT (#9272), anti-C/EBPα (#2295), anti-PI3K (#4257) and anti-PPARγ (#2443) antibodies from Cell Signaling Technology (Leiden, Netherlands); rabbit anti-adiponectin antibody (#GTX112777) from GeneTex Inc. (Ivrine, CA, United States); hFAB rhodamine anti-tubulin (#12004166) and anti-GAPDH (#12004168) antibodies and goat anti-rabbit IgG StarBright Blue 700 (#12004162) secondary antibody from Bio-Rad.