Ckx53 inverted microscope

For morphology analysis, 5 x 10⁵ cells were seeded in 6 well plates, and pictures were taken after 24-36 hours with an Olympus CKX53 inverted microscope. For the scratch wound healing assay, 2 x 10⁶ cells were seeded in 24-well plates. After 24 h, a pipette tip (1000 μl) was used to create a scratch wound. Next, the cell cultures were washed using PBS and incubated at 37C and 5% CO₂. The wounds were observed under an Olympus CKX53 inverted microscope for 24 h in situ to compare the number of cells that migrated into the wound areas.

Cells were observed and photographed using an OLYMPUS CKX53 inverted microscope (Olympus, Tokio, Japan) or an Invitrogen^TM EVOS^TM XL Core Imaging System (Thermo Fisher Scientific).

MCF-7 cells (5 × 10⁴ cells/well) were seeded in 24 well plates, overnight. After incubation, the cells were then exposed to various concentrations of As₂O₃ (0, 4, 11, 16 and 32 µM), cobalt chloride (50 and 100 µM) and curcumin (50 and 100 µM) for 24 h. After treatment, the cells were washed with sterile 1 × PBS and fixed with 3.7% paraformaldehyde for 10 min at room temperature. The cells were then washed twice with sterile 1 × PBS and stained with DAPI (5 µg/mL) for 15 min in the dark, at room temperature. Following incubation, the cells were washed twice with sterile 1 × PBS. Morphological changes of MCF-7 cells in each group were observed under the Olympus CKX53 Inverted Microscope, (Olympus, Shinjuku, Tokyo, Japan) and Eclipse Ti-U fluorescence microscope (Nikon Instruments Inc., Melville, NY, USA), for light and fluorescence microscopy, respectively. The images were captured with DSRI-1 camera and an LC-30 camera set, respectively.