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7 protocols using polyethylene terephthalate pet film

1

Nanofibrillated Cellulose Hydrogel Preparation

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Nanofibrillated cellulose hydrogel (2%) was prepared as previously reported using a mechanical homogenization method [37 ,38 (link)]. Nanocrystalline cellulose hydrogel (11.9%) was purchased from the Process Development Center, University of Maine. m-Cresol, lithium chloride, N,N-dimethylacetamide, chlorobenzene, phenol, polyethylenimine (PEI) (number average molecular mass Mn = 60 kg/mol), maleic acid (MA), sodium hypophosphite, glycidyl methacrylate (GMA, 97%), azoisobutyronitrile (AIBN), oligo (ethylene glycol) methyl ether methacrylate (OEGMA, Mn = 950 g/mol, stabilized with inhibitors), and inhibitor removers (kits for removing hydroquinone and monomethyl ether hydroquinone (MEHQ) and tert-butylcatechol (BHT)) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Silicon wafers (100 crystal plane) with a native oxide layer were purchased from University Wafer (South Boston, MA, USA). A polyethylene terephthalate (PET) film (0.50 mm thick) and a nylon 6,6 (PA 6,6) film (0.5 mm thick) were purchased from GoodFellow, Coraopolis, PA, USA. A cellophane film (regenerated cellulose) 0.03 mm thick was provided by Thermetrics. Cotton (100%, plain weave), nylon 6,6 (100%, spun, plain weave), cotton/polyester, PET (50%/50%, plain weave), polyester, and PET (100%, spun, plain weave) fabrics were purchased from Testafabrics, Inc., West Pittston, PA, USA.
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2

Thin-Film Deposition Protocols

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NaCl was purchased from ACROS Organics (99,5%, 207790010). Ultra-pure water was produced from a Q-grad®-1 MilliQ system (Millipore). Poly(Ethylene Terephthalate) (PET) film (thickness 13 μm, biaxial orientation) was purchased from Goodfellow (ES301061). Diethyl Zinc (DEZ) (Zn(CH2CH3)2, 95% purity, CAS: 557-20-0) and Trimethyl aluminum (TMA) (Al(CH3)3, 97% purity, CAS:75-24-1), were purchased from Sterm Chemical. Hexamethyldisilazane (HMDS) (reagent grade, ≥99%), was obtained from Sigma Aldrich
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3

Engineered Biomaterials for Tissue Regeneration

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Poly(dimethylsiloxane) (PDMS) pre-polymer (Sylgard 184) and a curing agent were purchased from Dow Corning (Midland, MI, USA). Tris-HCl was purchased from Biosesang (Seongnam, Korea). Dopamine hydrochloride and Alizarin Red S were obtained from Sigma-Aldrich (St. Louis, MO, USA). MSCs, HUVECs, MSC growth medium (MSCM), endothelial cell growth medium (ECM), endothelial cell growth supplements (ECGS), MSC growth supplements (MSCGS), fetal bovine serum (FBS), penicillin/streptomycin (P/S) solution, trypsin-EDTA (T/E) solution, Dulbecco’s phosphate-buffered saline (DPBS), MSC chondrogenic differentiation medium (MCDM), MSC chondrogenic differentiation supplement (MCDS), MSC osteogenic differentiation medium (MODM), and MSC osteogenic differentiation supplement A and B (MODS-A and MODS-B) were purchased from ScienCell (Carlsbad, CA, USA). Transforming growth factor-β3 (TGF-β3) and live/dead viability/cytotoxicity kits were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Safranin O was obtained from Polyscience (Warrington, PA, USA). Norland optical adhesive63 (NOA63) was purchased from Norland Products (Jamesburg, NJ, USA). Polystyrene (PS) sheets and poly(ethylene terephthalate) (PET) film were obtained from Goodfellow (Pittsburgh, PA, USA).
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4

PET Film Characterization and Functionalization

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Poly(ethylene terephthalate) (PET) film (thickness 13 µm, biaxial orientation) was purchased from Goodfellow (ES301061). Sodium chloride (71380), potassium chloride (P3911), sodium hydroxide (30603), hydrogen chloride (30721), branched PEI (Mw 25 kD, 408727), PAA (Mw 100 kD, 523925), PLL (30 kD-60kD P2636) were purchased from Sigma-Aldrich. Ultra-pure water was produced from a Q-grad®-1 Milli-Q system (Millipore).
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5

Diverse Substrate-Based Droplet Protocols

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The substrates used to support the different droplets include polyethylene terephthalate (PET) films, purchased from Goodfellow Corporation (Coraopolis, PA USA), petri dishes, purchased from USA Scientific (Ocala, FL USA), 6-well plates, purchased from USA Scientific (Ocala, FL USA), and silicon wafers, purchased from University Wafer (South Boston, MA USA).
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6

Biofilm Formation on Biomaterial Surfaces

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Staphylococcus aureus (methicillin resistant, ATCC 43300), Staphylococcus epidermidis (ATCC 49134) and Escherichia coli (ATCC 25922), were purchased from the American Type Culture Collection (ATCC, Manassas, VA USA. Human fetal osteoblasts (HfOB 1.19) were also purchased from the ATCC (Manassas, VA USA). The substratum biomaterials used to grow biofilms included polyethylene terephthalate (PET) films, purchased from Goodfellow Corporation (Coraopolis, PA USA), 6-well plates, purchased from USA Scientific (Ocala, FL USA) and silicon wafers, purchased from University Wafer (South Boston, MA USA).
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7

Fabrication of PET Microfluidic Devices

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Corning plain microscope slides (75 × 50 mm size, 1 mm thickness) were purchased from Fisher Scientific and thoroughly cleaned using piranha etch (a mixture of sulfuric acid, hydrogen peroxide and water). Polyethylene terephthalate (PET) films ( t=100±5μm , E=2-4GPa , and ν0.4 ) were purchased from GoodFellow and thoroughly cleaned using a standard degrease process. SU-8 2100 and SU-8 2000 thinner were purchased from Microchem Corp. and mixed to result in desired film thicknesses. Photoresist AZ4620 and Developer AZ 400 K were purchased from Integrated Micro Materials. Buffered oxide etchant (BOE; HF(48%):NH4F(40%):HCl(37%) = 1:6:1.4 by vol.) was purchased from MicroChemicals GmbH. Sylgard 184 silicone elastomer was purchased from Dow Corning for polydimethylsiloxane (PDMS) fluidic interconnects.
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