Crc 55tpet dose calibrator

All reagents were purchased from commercial suppliers and used without further purification. H-Asp (OtBu)-H NovaSyn TG resin (0.21 mmol/g) was obtained from Merck (Singapore). Fmoc-amino acids, HATU and HOAt, were obtained from Advanced Chemtech (Sabah, Malaysia). Fmoc-glutamic acid was t-butyl protected. (p-SCN-Bn)-NOTA was purchased from Macrocyclics (Plano, TX, USA). Sep-Pak^® light (46 mg) Accell™ plus QMA carbonate cartridges and Sep-Pak^® C18 light cartridges were purchased from Waters Corporation (Milford, CT, USA). Saline solution (0.9% w/v) was purchased from Braun Medical Industries (Singapore). All other chemicals and reagents were purchased from Sigma-Aldrich (Singapore), Fisher Scientific (Waltham, MA, USA) and Tokyo Chemical Industry (Chuo-ku, Tokyo, Japan). Carrier free aqueous [¹⁸F] fluoride was produced via the [¹⁸O(p,n)¹⁸F] nuclear reaction (GE PETtrace 860 cyclotron, Boston, MA, USA). Quality control of radiolabelled tracer was performed on a UFLC Shimadzu HPLC (Kyoto, Japan) system equipped with a dual-wavelength UV detector and a PMT-radio detector (Flow-Ram, Lab Logic, Broomhill, Sheffield, UK). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, Florham Park, NJ, USA).

The synthesis of NOTA–β-Ala-Gly-Gly-Ile-Glu-Phe-Asp-CHO (mNOTA-GZP) and characterisation details have been described previously [16 (link)]. The radiosynthesis of [¹⁸F]AlF-mNOTA-GZP has also been described previously [16 (link)]. No-carrier-added aqueous [¹⁸F]fluoride ion was produced via the [¹⁸O(p,n)¹⁸F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual-wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA). [¹⁸F]AlF-mNOTA-GZP was formulated as a colourless solution of 10% ethanol in saline (pH = 7.4) with a nondecay corrected radiochemical yield of 12-18%, a radiochemical purity of 99%, and molar activity of 50-81 GBq/μmol (n = 3) after a total reaction and purification time of 50 minutes.

H-Asp(OtBu)-H NovaSyn TG resin (0.21 mmol/g) was obtained from Merck. Fmoc-amino acids, HATU and HOAt were obtained from Advanced Chemtech. Fmoc-glutamic acid was t-butyl protected. (p-SCN-Bn)-NOTA was purchased from Boc Sciences and Macrocyclics. Glacial acetic acid was purchased from JT Baker. Sep-Pak® light (46 mg) Accell™ plus QMA carbonate cartridges and Sep-Pak® C18 light cartridges were purchased from Waters Corporation. Saline solution (0.9% w/v) was purchased from Braun Medical Industries. All other chemicals and reagents were purchased from Sigma-Aldrich, Fisher Scientific, and Tokyo Chemical Industry. No-carrier-added aqueous [¹⁸F]fluoride ion was produced via the [¹⁸O(p,n)¹⁸F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual-wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA).