Glucose consumption, lactate production, and PFK activity were measured using the Glucose Assay Kit, the Lactate Assay Kit, and the Phosphofructokinase Activity Colorimetric Assay Kit (all from Biovision, catalog nos. K686, K607, and K776, Milpitas, CA) according to the manufacturer's instructions, respectively. Intracellular adenosine triphosphate (ATP) levels and fructose‐6‐phosphate (F6P) levels were determined in cell lysates using the ATP Bioluminescent Somatic Cell Assay Kit and Fructose‐6‐Phosphate Assay Kit (Sigma–Aldrich, catalog nos. FLASC and MAK020) following the manufacturer's instructions.
Fructose 6 phosphate assay kit
Manufactured by Merck Group
Sourced in United States
The Fructose-6-Phosphate Assay Kit is a laboratory tool used to quantify the levels of fructose-6-phosphate, an important intermediate in carbohydrate metabolism. The kit provides a simple, colorimetric method to measure fructose-6-phosphate concentrations in various biological samples.
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Lab products found in correlation
2 protocols using fructose 6 phosphate assay kit
1
Metabolic Profiling of Cell Cultures
2
Extracellular Flux Analysis of Glycolysis
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We used Seahorse Bioscience Extracellular Flux Analyzer to detect glycosis via measuring extracellular acidification rate (ECAR) in real time. Basal ECAR is indicated by ECAR in the presence of glucose. Maxi ECAR is indicated by ECAR in the presence of Oligomycin.
Cells were plated in six-well plates. Culture medium was collected at 12 h, 24 h, 36 h, and 48 h. We used glucose and lactate assay kit (Shanghai Rongsheng Biotech, Shanghai, China) to measure the content of glucose and lactate following manufacturer’s instructions.
The content of F6P was measured by using the fructose-6-phosphate assay kit (MAK020, Sigma, USA).
Cells were plated in six-well plates. Culture medium was collected at 12 h, 24 h, 36 h, and 48 h. We used glucose and lactate assay kit (Shanghai Rongsheng Biotech, Shanghai, China) to measure the content of glucose and lactate following manufacturer’s instructions.
The content of F6P was measured by using the fructose-6-phosphate assay kit (MAK020, Sigma, USA).
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