We used 8 week-old C57BL6N male mice (~23-30 g) in all experiments, except for electrophysiological recordings that were done in 21 day-old C57BL6N male mice (Charles River, Calco, Italy). Mice were maintained in SPF facilities at the Mario Negri Institute and housed at a constant room temperature (23°C) and relative humidity (60 ± 5%) with free access to standard food pellet (2018S, Envigo, Udine, Italy) or to CBZ-in-food and its control pellet (BioServe, F05572; Frenchtown, NJ, USA; Grabenstatter et al, 2007) and water, and with a fixed 12 h light/dark cycle. Mice were housed 5 animals per cage. After experimental manipulations (as reported below) each mouse was individually housed in the presence of environmental enrichment (i.e. toilet paper, straw).
Male c57bl 6n mice
Manufactured by Charles River Laboratories
Sourced in Germany, China, Japan, France, United States, Canada, Italy
Male C57BL/6N mice are a widely used inbred mouse strain. They are a common rodent model used in a variety of research applications.
Automatically generated - may contain errors
Lab products found in correlation
Male cd 1 mice, by Charles River Laboratories (4 mentions)
C57bl 6n female mice, by Charles River Laboratories (2 mentions)
Transaminase cii b test wako, by Fujifilm (1 mentions)
C57bl 6j male mice, by Janvier Labs (1 mentions)
1324 forti, by Altromin (1 mentions)
Bms303141, by Selleck Chemicals (1 mentions)
High fat diet (hfd), by Charles River Laboratories (1 mentions)
L name, by Charles River Laboratories (1 mentions)
D12451, by Research Diets (1 mentions)
Male wistar rats, by Charles River Laboratories (1 mentions)
Isoproterenol hydrochloride powder, by Merck Group (1 mentions)
I12l rs 13 mhz transducer, by GE Healthcare (1 mentions)
Nexion 350x, by PerkinElmer (1 mentions)
Vivid q portable ultrasound system, by GE Healthcare (1 mentions)
D12492, by Research Diets (1 mentions)
Ketalar, by Pfizer (1 mentions)
Rompun vet, by Bayer (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Tipifarnib, by Selleck Chemicals (1 mentions)
Tipifarnib, by Merck Group (1 mentions)
106 protocols using male c57bl 6n mice
1
Experimental Conditions for C57BL6N Mice
2
DHP-Insensitive Cav1.2 Mice Protocol
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Male C57Bl6/N mice (Charles River Laboratories) were used for the study. Cav1.2DHP−/− mice carry a mutation (T1066Y, Exon 24) in the DHP-binding site of the α1 subunit rendering Cav1.2 insensitive to DHPs (30 (link)). Mice were between 8 and 14 weeks old, group-housed, and maintained on a 12-hour light/dark cycle.
3
Liver Injury Induction in Mice
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Male C57BL/6N mice (approximately 7 weeks old; Charles River Laboratories Japan, Yokohama, Japan) were maintained under a 12 h light/ 12 h dark cycle, fed with a conventional CE-2 laboratory diet (CLEA Japan, Tokyo, Japan), and were provided with water ad libitum for 1 week for acclimatization. The mice were then intraperitoneally administered with PCN (100 mg/kg), followed 6 h later by CCl4 (0.5 mL/kg, 10% v/v, in corn oil). After 24 h, they were sacrificed, and the blood and liver samples were collected. Plasma alanine aminotransferase (ALT) activity was determined using the Transaminase CII-B-test Wako (FUJIFILM Wako Pure Chemical) following the manufacturer’s instructions. All animal experiments were approved by the committee for animal experiments at University of Shizuoka and conducted in accordance with the guidelines for animal experiments at University of Shizuoka.
4
Streptozotocin-Induced Diabetic Mouse Model
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Male C57BL6/N mice (Charles River, Wilmington, MA, USA) and wild-type/REDD1 knockout B6;129 mice23 (link) were administered either 50 mg/kg streptozotocin (STZ) or sodium citrate buffer for 5 consecutive days to induce diabetes. Diabetic phenotype was assessed with fasting blood glucose levels >250 mg/dL. Approximately 2 g/kg per day N-acetyl-l -cysteine (NAC) was administered via drinking water. All procedures were approved by the Penn State College of Medicine Institutional Animal Care and Use Committee.
5
In Vivo EEG Recording in Mice
6
C57BL/6N Mice Experimental Protocol
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Male C57BL/6N mice of 2–4 months of age (Charles River) were used for all experiments. All procedures were approved by the animal use and care committee of the Institute of Basic Medical Sciences, University of Oslo, and the Centre for Comparative Medicine, Oslo University Hospital.
7
Behavioral Study of C57BL/6N Mice
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Male C57BL/6N mice of at least 10 weeks of age (Charles River) were used for the experiments. The mice were housed on a 12-h light:12-h dark cycle (lights on at 8 AM), 1–4 mice per cage. All experimental groups contain at least 4 animals, which is in line with sample sizes in the literature. Adequate measures were taken to minimize pain and discomfort. Experiments were carried out in accordance with the guidelines published in the European Communities Council Directive of 24. November 1986 (86/609/EEC). All procedures were approved by the Animal Use and Care Committee of the Institute of Basic Medical Sciences, The Faculty of Medicine, University of Oslo.
8
Dietary Omega-3 Supplementation in Mice
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Male C57BL/6N mice (Charles River Laboratories, Sulzfeld, Germany) were obtained at the age of ~10 weeks. After arrival, mice were individually housed in cages and maintained at ~22 °C on a 12-h light/dark cycle (light from 6:00 a.m.), with ad libitum access to water and a standard diet (Chow; ~14 kJ/g; fat content ~3.6% (w/w); Rat/Mouse-Maintenance extrudate; ssniff Spezialdiäten GmbH, Soest, Germany). After one week of adaptation, the animals were transferred to a thermoneutral environment (~30 °C) and fed the following experimental diets: (i) a lard-based high-fat diet (LHF diet; ~21 kJ/g; fat content ~35% (w/w); product “DIO-60 kJ% fat (Lard)”, Cat. No. E15742-34; ssniff Spezialdiäten GmbH, Soest, Germany), (ii) a LHF-based diet supplemented with omega-3 PUFA-containing PLs (ω3PL diet), using krill oil (Rimfrost Sublime; EPA ~13%, DHA ~8%; Rimfrost AS, Ålesund, Norway), and (iii) a LHF-based diet supplemented with omega-3 PUFAs in the form of re-esterified TAGs (ω3TG diet), using the product Epax 3000 TG (EPA ~18%, DHA ~11%; Epax Norway AS, Ålesund, Norway). Experimental diets were prepared at the ssniff facility in Germany. The total content of EPA and DHA in both supplemented diets was ~30 mg/g diet. For details on macronutrient and FA composition of the experimental diets, see Supplementary Materials Tables S1 and S2 , respectively.
9
C57BL/6N Mice Housing and Care
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Male C57BL/6N mice (7–12 weeks, Charles River, Houston, TX) were used throughout this study. The mice were housed on a 12–12 h light–dark cycle with standard bedding and free access to food and water in animal facility accredited by the Association for the Assessment and Accreditation of Laboratory and Care International. All experimental procedures using animals were done according to the guidelines of the Institutional Animal Care and Use Committee at the University of Texas Medical Branch.
10
In-utero Electroporation Experiments with Transgenic Mice
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For animal studies with in-utero electroporation, timed pregnant mice were used at embryonic day 14 (E14). Male C57Bl/6 and female CD1 mice were allowed to mate overnight (Obtained from Charles River).
In some experiments, wild type CD1 females were bred with homozygous Parvalbumin (Pv)-Cre (Pvalbtm1(cre)Arbr, JAX stock #017320) or heterozygous somatostatin (SST)-IRES-Cre (SSTtm2.1(cre)Zjh, JAX stock #018973) males.
For proteomic analysis, Male C57Bl6/N mice (Charles River) were used.
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