Stemi sv6

Manufactured by Zeiss

Sourced in Germany

The Stemi SV6 is a stereo microscope designed for industrial and lab applications. It features a binocular observation tube, a magnification range of 6x to 40x, and a working distance of 114 mm. The Stemi SV6 is equipped with LED illumination and offers high-quality optics for detailed observations.

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Lab products found in correlation

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Spelling variants of this product

Stemi SV6 stereomicroscope (5 citations) Stemi SV6/AxioCam MRc5 (2 citations) Stemi SV6 microscope (2 citations)

38 protocols using stemi sv6

Dental Caries Scoring Using Stereoscopic Magnification

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The teeth were prepared for caries scoring according to Larson’s modification of Keyes system [34]. One calibrated examiner performed the caries score of the codified jaws. The smooth surface caries scoring (buccal, morsal, proximal, lingual and palatinal) was done using a stereoscopic Zeiss magnifying lens (Stemi SV 6).
Before performing caries scoring on sulcal surfaces, the samples were prepared as follows: The jaws were painted with transparent nail polish to strengthen the structure and left to dry for 24 h. Murexide (SIGMA M-2628) dye solution (0.24 mg/mL) was used to stain the jaw and left to dry for 18 h. A sagittal cut was made in the jaws with a carbide disk coupled to a micromotor (mc52 – dent) to allow for better visualization of the sulcal surface. Caries scoring was performed on the sulcal surfaces using a stereoscopic Zeiss magnifying lens (Stemi SV 6).

Castillo Pedraza M.C., Rosalen P.L., de Castilho A.R., Freires I.D., de Sales Leite L., Faustoferri R.C., Quivey Jr R.G, & Klein M.I. (2019). Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo. Journal of Oral Microbiology, 11(1), 1607505.

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Quantifying Arabidopsis Root Development

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Arabidopsis primary root lengths and the number of lateral roots were recorded from 10-day-old seedlings vertically cultured on 0.5× MS with 1% sucrose. Primary root lengths were measured using ImageJ software (v1.8.0_172). Pericycle activation for lateral root initiation was performed according to the method described in Himanen et al.^{21 (link)}. Specifically, 4-day-old Arabidopsis seedlings vertically cultured on 0.5× MS were transferred to vertical square plates containing 0.5× MS and 10 μM NPA. On the 3rd day of culturing on NPA, they were transferred onto 0.5× MS plus 10 μM NAA. To observe root hair morphology on NPA, seedlings grown on NPA remained growing on the same medium after the 3rd day and images of root hairs were taken on the 6th day of culturing on NPA. Roots were photographed under the Stereo Dissecting Microscope (Zeiss Stemi SV6). The number and lengths of root hairs were measured using ImageJ software (v1.8.0_172). Statistical analysis was performed using GraphPad Prism (v9.2.0).

Zhang M., Bodi Z., Mackinnon K., Zhong S., Archer N., Mongan N.P., Simpson G.G, & Fray R.G. (2022). Two zinc finger proteins with functions in m6A writing interact with HAKAI. Nature Communications, 13, 1127.

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Inhibition of Tumor Angiogenesis by KGP94

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All in vivo experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Florida. Induction of angiogenesis by breast cancer MDA-MB-231 cells and the ability of KGP94 to inhibit tumor angiogenesis in vivo was measured by performing intradermal assay as described previously [27 (link)]. 5 × 10⁵ parental or CTSL knockdown MDA-MB-231 cells were injected intradermally on the ventral surface of athymic NCR female nu/nu mice at four sites. To facilitate easy location of the site of tumor cell inoculation, one drop of trypan blue solution was added to impart a light blue color to the cell suspension. 10 or 20 mg/kg KGP94 was administered intra-peritoneally on a daily basis. 3 days later, the mice were euthanized and their skin flaps were removed and promptly analyzed. Tumor angiogenesis was evaluated by counting the number of blood vessels growing into the tumor nodule using a Zeiss Stemi SV 6 dissecting microscope. Tumor nodule images were captured using a Leica MZ 16 F camera and Leica Application Suite software.

Sudhan D.R., Rabaglino M.B., Wood C.E, & Siemann D.W. (2016). Cathepsin L in tumor angiogenesis and its therapeutic intervention by the small molecule inhibitor KGP94. Clinical & experimental metastasis, 33(5), 461-473.

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Imaging Protocol for Microscopy Techniques

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For plate imaging, a Nikon D7000 camera was used with an AF Micro Nikkor 60-mm macro lens. The stereomicroscope was a Zeiss Stemi SV6 stereomicroscope equipped with a 1.0× Achromat S objective lens and an AxioCam charge-coupled device (CCD) video camera system (Zeiss). For imaging, we used Axiovision suite software from Zeiss. The upright microscope was a Zeiss Axioskop 2 Plus equipped with an A-Plan 10× objective and a long-distance Plan-NEOFLUAR 20× objective. The camera was an AxioCam MRc (Zeiss) with Axiovision software to capture images. The inverted microscope was a Nikon Eclipse TE2000-U microscope equipped with a 20× Plan Apo objective and a 60× Plan Apo oil objective. Pictures were made with a Hamamatsu digital camera, model ORCA-ER. Filter sets were from Chroma, model #52017 (CFP-YFP dual-band filter), and model #62002v2 (DAPI/FITC/Texas Red).

van Gestel J., Vlamakis H, & Kolter R. (2015). From Cell Differentiation to Cell Collectives: Bacillus subtilis Uses Division of Labor to Migrate. PLoS Biology, 13(4), e1002141.

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Multimodal Imaging of Biological Samples

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In vivo images were acquired with Scmex 3.0 camera (DC.3000s, Visual Inspection Technology) in a Zeiss Stemi SV 6 binocular loupe. Brightfield colorimetric ISH images obtained with a ProgRes C3 camera from Jenoptik (Jena, TH, Germany). A Zeiss LSM 880 confocal microscope (Zeiss, Oberkochen, Germany) was used to obtain confocal images of whole-mount immunostainings. Fiji/ImageJ^{120 (link)} was used to show representative confocal stacks for each experimental condition.

Pascual-Carreras E., Marín-Barba M., Castillo-Lara S., Coronel-Córdoba P., Magri M.S., Wheeler G.N., Gómez-Skarmeta J.L., Abril J.F., Saló E, & Adell T. (2023). Wnt/β-catenin signalling is required for pole-specific chromatin remodeling during planarian regeneration. Nature Communications, 14, 298.

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Dietary Habits of Symbiotic and Free-Living Crabs

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We analyzed the stomach contents of L. ferreirae sampled from February 2013 to May 2014. The crabs were classified into the following 4 demographic categories: AS—adult symbiont; JS—juvenile symbiont; AF—adult free-living; JF—juvenile free-living. Stomachs were removed and fixed in 70% alcohol and observed with either a light microscope (Zeiss^® Axioskop 2 plus) or stereomicroscope (Zeiss^® Stemi SV6), equipped with a digital imaging system (Zeiss Stemi 2000-C; precision = 0.001 mm). The identification of food items was performed according to Mariscal (1974) , Barros et al. (2008) , and Gonçalves et al. (2020a ).
We used a modified version of the quantitative scoring procedure from Williams (1981) and Mantelatto and Christofoletti (2001) to analyze the food items in the crab’s stomach. We evaluated the relative contribution of each food category (in relation to the total volume of each stomach) assigning a classification score from 1 to 10: 1 = contribution of 0–10% of the stomach’s volume, 2 = contribution of 10–20% of the stomach’s volume, 3 = contribution of 20–30% of the stomach’s volume, and so on.

Gonçalves G.R., Wolf M.R., Antunes M., Amorim F.W., Negreiros-Fransozo M.L, & Leão Castilho A. (2021). Ontogenetic niche specialization of the spider crab Libinia ferreirae associated with the medusa Lychnorhiza lucerna. Current Zoology, 68(5), 549-559.

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Antitumor Effects of KGP94 on PC-3ML Xenografts

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1 × 10⁵ PC-3ML cells were injected intradermally at four sites on the ventral surface of athymic NCR nu/nu male mice. One drop of trypan blue solution was added to impart a light blue color to the cell suspension and thus allow easy location of the site of cancer cell inoculation. A dose of 10 or 20 mg/kg KGP94 was administered IP on a daily basis. Three days later, the mice were euthanized and their skin flaps were removed and promptly analyzed. Tumor angiogenesis was evaluated by counting the number of blood vessels growing into the tumor nodule using a Zeiss Stemi SV 6 dissecting microscope.^{25 (link)} Tumor nodule images were captured using a Leica MZ 16 F camera and Leica Application Suite software.

Sudhan D.R., Pampo C., Rice L, & Siemann D.W. (2016). Cathepsin L inactivation leads to multimodal inhibition of prostate cancer cell dissemination in a preclinical bone metastasis model. International journal of cancer, 138(11), 2665-2677.

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Sexual Dimorphism in Mating Insects

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During mating, 20 males and 20 females were identified according to their position in copula. Individuals of each sex were then sacrificed by freezing and the external structures were observed under Zeiss Stemi SV6 stereoscope at 40 × magnification with the aid of an ocular micrometer, searching for sexual differentiation characters. The identified material was deposited in the entomological collection of the UEMS.

Rodrigues S.R., Gomes E.S, & Bento J.M. (2014). Sexual Dimorphism and Mating Behavior in Anomala testaceipennis. Journal of Insect Science, 14, 210.

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GUS Staining and Microscopy of Transgenic Plants

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Positive transgenic plants were identified using GUS staining as described (Jefferson et al., 1987 (link)). Pictures were taken using a digital camera (G12, Canon) or microscope (STEMI SV6, Zeiss) equipped with a digital camera.

Dai C., Lee Y., Lee I.C., Nam H.G, & Kwak J.M. (2018). Calmodulin 1 Regulates Senescence and ABA Response in Arabidopsis. Frontiers in Plant Science, 9, 803.

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Monitoring Asian Tiger Mosquito Populations

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The population dynamics was monitored weekly using a network of 15 ovitraps set up on June 21^st 2010 [34 (link)]. Ovitraps are artificial containers made up of 3 L black plastic buckets filled with 2 L of tap water changed weekly. In each container a floating polystyrene square (5 x 5 cm) is added to provide a support for oviposition. Ovitraps were hidden under vegetation and egg batches were collected weekly and brought back to laboratory for counting. The collection was stopped in November after two consecutive negative reports, marking the end of the active season of the Asian tiger mosquito. In 2011 and 2012 the network was reinstalled from the end of March and was expanded to 18 ovitraps. The surface of the trapping area was computed as the surface of the smallest polygon including all ovitraps with a buffer distance of 50 m, and covered 30.5 hectares in 2010 and 41.6 ha the following years. Both hatched and unhatched eggs of A. albopictus collected in ovitraps were counted in laboratory under a stereomicroscope Zeiss Stemi SV6.

Lacour G., Chanaud L., L’Ambert G, & Hance T. (2015). Seasonal Synchronization of Diapause Phases in Aedes albopictus (Diptera: Culicidae). PLoS ONE, 10(12), e0145311.

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