Arginylglycylaspartic acid

The following reagents were obtained from the respective suppliers: phorbol-12-myristate 13-acetate (PMA; Cayman Chemical (Ann Arbor, MI, USA)); 1,25-dihydroxy vitamin D3 (Toronto Research Chemicals, Inc., Toronto, ON, Canada); Roswell Park Memorial Institute (RPMI)-1640, Dulbecco modified Eagle medium (DMEM), Dulbecco phosphate-buffered saline (DPBS), and fetal bovine serum (FBS) (Welgene, Inc., Daegu, Republic of Korea); Interferon-gamma (IFN-γ) and anti-MER-TK antibodies (R&D Systems, Minneapolis, MN, USA); Arginylglycylaspartic acid (RGD), phytohemagglutinin P (PHA), and LPS from Escherichia coli K12 (Sigma-Aldrich, St. Louis, MO, USA); carboxyfluorescein succinimidyl ester (CFSE) CellTrace™ Cell Proliferation Kit, and antibiotic-antimycotic (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA); ethylenediaminetetraacetic acid (EDTA; Amresco, Inc., Solon, OH, USA); Galα1-3Galβ1-4GlcNAcβ-PAA trisaccharide (α-Gal; GlycoTech, Gaithersburg, MD, USA); anti-CD14, anti-CD274, and anti-CD80 (eBioscience Inc. (San Diego, CA, USA); anti-CD16 (Biolegend Inc., San Diego, CA, USA); anti-DHRS9 (Abnova, Taiwan, China); anti-CD11b (BD Biosciences Inc., Franklin Lakes, NJ, USA); anti-CD209 (Serotec Bio-Rad Inc., CA, USA); and FITC-conjugated, in addition to other antibodies (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Iron(II) chloride tetrahydrate (FeCl₂·4H₂O, 99–102%; J. T. Baker), Iron(III) chloride hexahydrate (FeCl₃·6H₂O; Merck), Hydrochloric acid (HCl, 37%; Fluka), Ammonia solution (25%,Merck), sodium iron chlorophyllin salt (Chl/Fe), H₂N-PEG-NH₂ (MW = 3500; JenKem Technology), 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, 99%; Matrix scientific), N-hydroxysuccinimide (NHS, 98%+; Alfa Aesar), 4-carboxyphenylboronic acid (HO₂CC₆H₄B(OH)₂; Aldrich), hydrogen peroxide (H₂O₂, 34.5–36.5%, Sigma-Aldrich), 3,3′,5,5′-Tetramethylbenzidine (TMB, 99%, Sigma-Aldrich) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay reagent, GoldBio), 2,7-dichlorofluorescin diacetate (DCFH-DA, ≥ 97% Sigma-Aldrich), transferrin (≥ 98%, Sigma-Aldrich), Arginylglycylaspartic acid (RGD, ≥ 97% Sigma-Aldrich), N,N-dimethyl-4-nitrosoaniline (RNO, 98%, Alfa Aesar), Imidazole (99%, Acros organics), dimethyl sulfoxide (DMSO, ≥ 99.7%, Fisher scientific), Cytotoxicity LDH Assay Kit purchased from dojindo, Glutathione Fluorescent Detection Kit purchased from ThermoFisher, Cell medium (DMEM, RPMI-1640, McCoy’s 5A, F12K) purchased from Corning.