MDA-MB-231 (human breast adenocarcinoma) and LNCaP cells were obtained from American Type Culture Collection (ATCC). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM l -glutamine, and 1% penicillin-streptomycin (v/v). LNCaP cells were propagated in RPMI-1640 (Sigma-Aldrich), supplemented with 10% FBS, 1% penicillin/streptomycin and 1% l -glutamine.
Lncap cells
Manufactured by Merck Group
Sourced in Germany, United States
LNCaP cells are a human prostate cancer cell line derived from a lymph node metastasis. They are widely used in cell biology and cancer research as a model system for studying prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Lncap cell, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a modified medium, by Thermo Fisher Scientific (1 mentions)
Ht 29 cell, by American Type Culture Collection (1 mentions)
Rpmi medium, by Merck Group (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Apalutamide, by Selleck Chemicals (1 mentions)
Dihydrotestosterone dht, by Merck Group (1 mentions)
Charcoal stripped fbs, by Thermo Fisher Scientific (1 mentions)
Hek293 cells, by Merck Group (1 mentions)
5α androstan 17β ol 3 one, by Merck Group (1 mentions)
Enzalutamide, by Selleck Chemicals (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Huvecs, by Merck Group (1 mentions)
Nodal, by Merck Group (1 mentions)
5 protocols using lncap cells
1
Cell Culture Protocol for MDA-MB-231 and LNCaP
2
Prostate Cell Line Characterization
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The human prostate cell lines used in this study were LNCaP and VCaP. Both cell lines were maintained in standard growth medium, supplemented with 10% fetal bovine serum (Gibco by Life Technologies, Carlsbad, CA, USA) and 1% penicillin/streptomycin solution (Gibco) in a humidified chamber (37°C, 5% CO ). LNCaP cells were acquired from the German Resource Centre for Biological Material (DSMZ, Braunschweig, Germany) and VCaP cells from the European Collection of Cell Cultures (Sigma-Aldrich, St Louis, MO). For validation purposes, both prostate cell lines were karyotyped by G banding and probed for ERG and ETV1 rearrangements by FISH analysis. Cultures were considered Mycoplasma-free by routine testing for Mycoplasma spp. contamination (PCR Mycoplasma Detection Set; Clontech Laboratories Inc., Mountain View, CA, USA).
3
Cell Line Sourcing and Cultivation
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8505c cells were purchased from ECACC (cat. no. 94090184), LNCaP cells were purchased from Sigma-Aldrich (cat. no. 89110211), SW1736 cells were purchased from CLS (cat. no. 300453). Hth104, KTC1 and TPC-1 cells were a gift from Prof. James Fagin (MSKCC, New York, USA) and were cultured in RPMI medium (Sigma-Aldrich, cat. no. R8758). K1 cells were a gift from Prof. James Fagin (MSKCC, New York, USA) and cultured in DMEM:F12 medium (Gibco, cat. no. 11320–074). HT-29 cells were purchased from ATCC (cat. no. HTB-38) and cultured in Modified McCoy’s 5A medium (Gibco, cat. no. 26600–080). Media of all cell lines were supplemented with 10% FBS (Gibco, cat. no.10270), 100 U/ml penicillin (Sigma-Aldrich, cat. no. P0781), 0.1 mg/ml streptomycin (Sigma-Aldrich, cat. no. P0781). Media of 8505c, LNCaP and K1 cells were additionally supplemented with 1X Non-Essential Amino Acids (NEAA) (BioConcept, cat. no. 5-13 K00). All cell lines were kept up to 50 passages or 6 months, whichever limit was reached first.
4
Androgen receptor regulation via LSD1 inhibitors
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Cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (HEK293 cells; Sigma) or Roswell Park Memorial Institute (RPMI) medium (LNCaP cells; Sigma) supplemented with 10% fetal bovine serum (FBS) with 4 mM (HEK293) or 2 mM (LNCaP) l -glutamine and 1 mM pyruvate. For experiments, LNCaP cells were incubated for 24 h and then media was changed to RPMI-1640 (phenol free; Life Technologies) supplemented, for the remainder of the experiment, with 10% charcoal stripped FBS (Life Technologies). Dihydrotestosterone (DHT) and 5α-Androstan-17β-ol-3-one were from Sigma-Aldrich; LSD1 inhibitors LSD1-C76 and HCI-2509 were from Xcess Biosciences; enzalutamide and apalutamide were from Selleckchem. Chemical inhibitors of LSD1 were synthesised, and a separate publication will describe full details of the preparation.
5
Prostate Cancer's Impact on Angiogenesis
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Human umbilical vein endothelial cells (HUVECs) and the human prostate cancer DU145 cells and LNCaP cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). RPMI 1460 medium (Gibco, Waltham, MA, USA) supplemented with 10% foetal bovine serum (HyClone, Logan, UT) and 1% penicillin–streptomycin (Gibco, Waltham, MA, USA) was used for cell culture at 37 °C in 7% CO2 for 3–4 days. To study the relationship between the Nodal/ALK4 pathway and angiogenesis, both cell lines were treated with Nodal (Sigma, St Louis, MO, USA) for 8 h at a concentration of 5 μM. To explore the effect of prostate cancer on angiogenesis, HUVECs were co-cultured with DU145 cells and LNCaP cells.
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