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Is200

Manufactured by Thorlabs
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Sourced in United States

The IS200 is a power meter that measures optical power in the wavelength range of 400 to 1100 nm. It features a large, easy-to-read display and provides accurate power measurements in both continuous wave and pulsed modes.

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Lab products found in correlation

Superk extreme, by NKT Photonics (1 mentions) Sigma vp, by Zeiss (1 mentions) Axio imager a2m, by Zeiss (1 mentions) Vertex 70v, by Bruker (1 mentions) Qth10, by Thorlabs (1 mentions) Ocean hdx vis nir, by OceanOptics (1 mentions) M365lp1, by Thorlabs (1 mentions) Avalight hal, by Avantes (1 mentions) Avaspec 2048, by Avantes (1 mentions)

7 protocols using is200

1

Absolute Quantum Yield Measurement of NIR-IIb Probes

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The absolute quantum yield measurement was performed by following a literature protocol53 with slight modifications. The NIR-IIb probes were excited by a 980 nm laser (for Zn doped α-ErNPs and β-ErNPs) or an 808 nm laser (for PbS quantum dots). The laser power density was 100 mW/cm2. An integrating sphere (Thorlabs; IS200) was used to spread the incoming light by multiple reflections over the entire sphere surface. The outcome lights, including laser excitation light and NIR fluorescence of NIR-IIb probes, were taken using a home-built NIR spectroscopy with a spectrometer (Acton SP2300i) equipped with a liquid-nitrogen cooled InGaAs linear array detector (Princeton OMA-V). Note that, the excitation light has to be attenuated by a neutral density filter (Newport; OD = 2.0) before being detected. According to the equation (1), the absolute quantum yield of NIR-IIb probes was calculated.
QY=photonsemittedphotonsabsorbed=E[sample]L[blank]−L[sample]
Where QY is the quantum yield, E[sample] is the emission intensity, L[blank] and L[sample] are the intensities of the excitation light in the presence of the water and the NIR-IIb probe sample, respectively.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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2

Absolute Quantum Yield Measurement of NIR-IIb Probes

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The absolute quantum yield measurement was performed by following a literature protocol53 with slight modifications. The NIR-IIb probes were excited by a 980 nm laser (for Zn doped α-ErNPs and β-ErNPs) or an 808 nm laser (for PbS quantum dots). The laser power density was 100 mW/cm2. An integrating sphere (Thorlabs; IS200) was used to spread the incoming light by multiple reflections over the entire sphere surface. The outcome lights, including laser excitation light and NIR fluorescence of NIR-IIb probes, were taken using a home-built NIR spectroscopy with a spectrometer (Acton SP2300i) equipped with a liquid-nitrogen cooled InGaAs linear array detector (Princeton OMA-V). Note that, the excitation light has to be attenuated by a neutral density filter (Newport; OD = 2.0) before being detected. According to the equation (1), the absolute quantum yield of NIR-IIb probes was calculated.
QY=photonsemittedphotonsabsorbed=E[sample]L[blank]−L[sample]
Where QY is the quantum yield, E[sample] is the emission intensity, L[blank] and L[sample] are the intensities of the excitation light in the presence of the water and the NIR-IIb probe sample, respectively.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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3

Reflectance and Transmission Spectra Characterization

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The visible and NSWIR photographs of the samples were taken using Nikon D3300 camera and NIRvana ST 640 camera, respectively. Microscopy was performed using the Zeiss Axio Imager.A2m optical microscope and Zeiss Sigma VP scanning electron microscope. The reflectance spectra were taken separately in two wavelength ranges: visible to near-infrared (0.4 to 1.0 μm) and near-infrared to LWIR (1.0 to 15 μm) for incident angles of 30°. In the first range, the reflectance was measured using an integrating sphere (model IS200, Thorlabs) containing a silicon detector and coupled to a high-power supercontinuum laser (SuperK EXTREME, NKT Photonics) and a tunable filter (Fianium LLTF contrast). The sample was put inside the integrating sphere. A calibrated diffuse reflector (item SM05CP2C, Thorlabs) was used as the reference. In the second range, reflectance was measured using a gold integrating sphere (model 4P-GPS-020-SL, Labsphere) coupled with a mercury cadmium telluride detector and a Fourier transform infrared spectrometer (VERTEX 70v, Bruker). A gold-coated aluminum foil was used as the reference. The spectra in the two ranges were then patched to obtain the final reflectance. The transmission spectra were obtained in the same way, except that the sample was placed at the mouth of the integrating sphere.
Chen Y., Mandal J., Li W., Smith-Washington A., Tsai C.C., Huang W., Shrestha S., Yu N., Han R.P., Cao A, & Yang Y. (2020). Colored and paintable bilayer coatings with high solar-infrared reflectance for efficient cooling. Science Advances, 6(17), eaaz5413.
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4

Absolute Quantum Yield Measurement

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The absolute quantum yields of Au-GSH and Au-PC were measured using an integrated sphere (Thorlabs; IS200). The probes were excited by an 808 nm laser and the emission was collected in the 900 – 1500 nm. After spreading the incoming light by an integrated sphere, the outcome light was collected using a home-built NIR spectrograph with a spectrometer (Acton SP2300i) equipped with a liquid-nitrogen-cooled InGaAs linear array detector (Princeton OMA-V). The absolute quantum yields were calculated according to the following equation: QY=photonsemittedphotonsabsorbed=E[sample]Lblank−Lsample where QY is the quantum yield, E[sample] is the emission intensity, and L[blank] and L[sample] are the intensities of the excitation light in the presence of the water and the NIR-II probe sample, respectively.
Baghdasaryan A., Wang F., Ren F., Ma Z., Li J., Zhou X., Grigoryan L., Xu C, & Dai H. (2022). Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models. Nature Communications, 13, 5613.
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5

Optical Properties of Gelatin-HAP Composites

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Reflectance and transmittance measurements were collected from 1 mm slices of solidified gelatin mixtures, HAP and gelatin powder with an integrating sphere (IS) (IS200; Thorlabs; New Jersey; USA) illuminated using a quartz-tungsten-halogen (QTH) lamp (QTH10; Thorlabs; New Jersey; USA). Bulk optical properties ( μa,μs′ ) were calculated from reflectance and transmission measurements using the inverse adding-doubling (IAD) technique[31 ], which performs an iterative solution to the radiative transport equation. For the purpose of IAD, anisotropy was held constant at 0.75, and the refractive index was also set statically to 1.51 for the gelatin matrix medium, and 1.651 for the HAP capsule. Optical property spectra were nearly monotonic in the Raman fingerprint window for the 785 nm excitation wavelength evaluated here, and on a similar order to those reported in literature for soft tissues and bone[32 (link)] (835 to 915 nm for λ , 780-1800 cm−1 Raman Shifts). Mean optical property values were; Gelatin matrix μa=0.7cm−1μs′=1.6×10−7cm−1 , HAP μa=11cm−1μs′=770cm−1 , however the full spectra were input in the MC model.
Dumont A.P., Fang Q, & Patil C.A. (2021). A Computationally Efficient Monte-Carlo Model for Biomedical Raman Spectroscopy. Journal of biophotonics, 14(7), e202000377.
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6

Luminescence Quantum Yield Measurement

Cited 1 time
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The luminescence quantum yield measurement was conducted by following protocols established in previous reports (14 (link), 33 (link), 50 (link)). Specifically, the SMSO nanophosphor colloid was excited by a collimated 365-nm light beam coupled from an LED (M365LP1, Thorlabs, Newton, NJ). An integrating sphere (IS200, Thorlabs, Newton, NJ) and a nonscanning fiber-coupled spectrometer (OCEAN-HDX-VIS-NIR; Ocean Optics, Orlando, FL) were used to redirect and collect the excitation and emission light simultaneously both during and after the recharging. The absorbed photons were measured by replacing the SMSO colloid with water and repeating the above procedure. The luminescence quantum yield was then calculated as follows QY=Photons emittedPhotons absorbed=∫0tLumIEm dt(IEx_ref−IEx_SMSO) tEx where QY is the luminescence quantum yield; IEm is the emission light intensity; IEx_ref and IEx_SMSO are the excitation light intensity in the presence of water or SMSO nanophosphor colloid, respectively; and tLum and tEx are the duration of luminescence and excitation light, respectively.
Yang F., Wu X., Cui H., Ou Z., Jiang S., Cai S., Zhou Q., Wong B.G., Huang H, & Hong G. (2022). A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source. Science Advances, 8(30), eabo6743.
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7

Integrating Sphere Absorption Spectroscopy

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A schematic overview of the setup is shown in Fig. 1. An integrating sphere (Thorlabs IS200) with a radius of 25.4 mm and a sphere wall reflectance ρ of 0.99 was used in the setup. A polyethylene tube (Portex) with inner radius of 0.29 mm and outer radius of 0.48 mm was inserted through two opposite ports. The tube's axis was located approximately 5 mm above the center of the sphere. The absorber was injected into this tube. Injecting the absorber until it flowed out on the other side of the tube ensured that the tube was completely filled. A tungsten halogen light source (Avantes AvaLight-Hal) was used for illumination and was connected to a converging lens by an optic fiber (Thorlabs M37L01, core diameter of 550 μm, 0.22 NA). This ensured that incident light could not directly hit the tube and was at least reflected diffusively once before hitting the tube. The lens was connected to the input port of the IS. A fiber-optic spectrometer (Avantes AvaSpec-2048) was used for detection of the outgoing light and was connected to the output port of the IS by another optic fiber of the same type.
Villanueva Y., Veenstra C, & Steenbergen W. (2016). Measuring absorption coefficient of scattering liquids using a tube inside an integrating sphere. Applied optics, 55(11).
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