Alexa fluor 647 conjugated anti rabbit igg h l

Manufactured by Cell Signaling Technology

Sourced in United States

Alexa Fluor 647-conjugated anti-rabbit IgG (H+L) is a secondary antibody that specifically binds to rabbit immunoglobulin G (IgG) heavy and light chains. The antibody is conjugated to the Alexa Fluor 647 fluorescent dye, which can be detected using appropriate instrumentation.

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Lab products found in correlation

P atr, by Cell Signaling Technology (1 mentions) P cdc25c, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated anti mouse igg h l, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Alexa Fluor 647-conjugated anti-rabbit IgG Anti-rabbit IgG Alexa Fluor 647 Alexa 647 conjugated anti-rabbit IgG Alexa Fluor 647 Alexa 647 Rabbit anti-IgG IgG rabbit

2 protocols using alexa fluor 647 conjugated anti rabbit igg h l

Digitoxin Induces Apoptosis Signaling

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A library of 78 natural compounds was obtained from Target Molecule Corp. Digitoxin (≥98% pure) was purchased from Baoji Herbest Bio-Tech Co., Ltd. MTT was supplied by Sigma-Aldrich (Merck KGaA). An Annexin-V-FITC/propidium iodide (PI) staining assay kit was obtained from Beyotime Institute of Biotechnology. The bicinchoninic protein assay kit (BCA) was purchased from Thermo Fisher Scientific Inc., while PI and 4′,6-dimidyl-2-phenylindole (DAPI) were purchased from Roche Diagnostics (Shanghai) Co. Ltd. Primary antibodies against cyclin-dependent kinase 1 (CDK1, #9116), cyclin B1 (#4138), phosphorylated (p)-CDK1 (Thr14) (#2543), p-histone H2AX (γH2AX, #9718), ATR (#2790), p-ATR (Ser428) (#2853), CHK2 (#6334), p-Chk2 (Thr68) (#2197), CDC25C (#4688), p-CDC25C (Thr48) (#12028), Bax (#5023), Bcl-2 (#15071), cytochrome c (#11940), caspase-9 (#9508) and-3 (#9662), cleaved-caspase-3 (#9579) and −9 (#20750), cleaved poly (ADP-ribose) polymerase (PARP) (#5625), β-actin (#4970) and the horse-radish peroxidase (HRP)-conjugated secondary antibodies (Anti-mouse IgG, #7076; Anti-rabbit IgG, #7074), Alexa Fluor 647-conjugated Anti-rabbit IgG (H+L) (#4414) were obtained from Cell Signaling Technology Inc., (dilution of primary antibodies, 1:1,000; dilution of secondary antibodies, 1:2,000).

Lei Y., Gan H., Huang Y., Chen Y., Chen L., Shan A., Zhao H., Wu M., Li X., Ma Q., Wang J., Zhang E., Zhang J., Li Y., Xue F, & Deng L. (2020). Digitoxin inhibits proliferation of multidrug-resistant HepG2 cells through G2/M cell cycle arrest and apoptosis. Oncology Letters, 20(4), 71.

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Immunostaining Analysis of LC3 Expressions in Aortic Samples

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LC3 expressions were analyzed with immunostaining in aortas. The part of aorta with maximum diameter aortas (n = 8 per group) were harvested collected and embedded in Tissu-Tek O.C.T. compound (SAKURA 4583, SAKURA FINETEK USA INC.) The samples were frozen at −80 °C. Then, 5-μm sections were fixed in pre-cold 4% formaldehyde for 15–20 min. After fixation, samples were blocked in 5% BSA in PBS for 1 h. After blocking, samples were incubated with LC3B (E5Q2K) mouse mAb (#83506, Cell Signaling Technology, USA) and rabbit anti-alpha smooth muscle actin antibody (#ab124964, Abcam, Cambridge, USA) at 4 °C for 12 h. The secondary antibodies including Alexa, Fluor 488 conjugated anti-mouse IgG (H + L) (#4408, Cell Signaling Technology, Boston, MA, USA) and Alexa, Fluor 647 conjugated anti-rabbit IgG (H + L) (#4414, Cell Signaling Technology, Boston, MA, USA) were used for detection. Nuclei was stained with 40, 6-diamidino-2-phenyindole, DAPI (Vector Laboratories, Burlingame, CA, USA). ProLong™ Gold Antifade Mountant (Thermo Fisher, P36930) was applied to protect the fluorescence from fading. Images were taken using a Carl Zeiss Axio Imager AX10 with ZEN 2011 (blue edition). The fluorescence was measured using digital image analysis software (ImageJ v.1.41, National Institutes of Health).

Wu Q.Y., Cheng Z., Zhou Y.Z., Zhao Y., Li J.M., Zhou X.M., Peng H.L., Zhang G.S., Liao X.B, & Fu X.M. (2020). A novel STAT3 inhibitor attenuates angiotensin II-induced abdominal aortic aneurysm progression in mice through modulating vascular inflammation and autophagy. Cell Death & Disease, 11(2), 131.

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