I stat portable analyzer

Manufactured by Abbott

Sourced in United States

The I-STAT® portable analyzer is a compact, handheld device designed for point-of-care testing. It is capable of analyzing a variety of clinical samples, including blood, to provide rapid diagnostic information.

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Lab products found in correlation

Isoflurane, by Abbott (1 mentions) Rodent ventilator, by Harvard Apparatus (1 mentions) Cg4 cartridge, by Abbott (1 mentions)

3 protocols using i stat portable analyzer

Neonatal Lamb Vitality Assessment

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Complete physical examination was performed immediately after birth through neonatal Apgar
score of vitality adapted to lambs (
Vannucchi et al., 2012
), with an additional separate analysis of cardiopulmonary auscultation, heart rate (HR)
and respiratory rate (RR). Furthermore, lambs were evaluated for their rectal temperatures
at the same time that the Apgar assessment was performed.
Immediately after clinical evaluation, arterial blood samples were collected by femoral
artery puncture with heparinized sterile needles and syringes for hemogasometric analysis.
We used i-STAT^® portable analyzer (Abbott) with the EG7+ cartridge,
which provided the following data: pH, pO² (oxygen pressure - mmHg), pCO² (pressure of carbon dioxide - mmHg), TCO² (total carbon dioxide - mmol/L),
HCO^3- (bicarbonate - mmol/L), BE (base excess - mmol/L), SO² (oxygen
saturation - %), sodium (Na⁺ - mmol/L) and potassium (K⁺ - mmol/L).
Blood glucose concentration was determined in venous samples taken from jugular vein. A drop
of blood was inserted in a glucometer (Advantage Monitor^®, Roche Diagnostics)
and the result was expressed as mmol/L.

Silva L.C., Regazzi F.M., Lúcio C.F., Veiga G.A., Angrimani D.S., Fernandes C.B, & Vannucchi C.I. (2018). Redox, acid-base and clinical analysis of preterm and term neonatal lambs. Animal Reproduction, 15(1), 51-55.

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Lung Transplantation in Rats

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Recipient Sprague-Dawley rats (260–300g, Charles River) were anesthetized with 5% isoflurane (Abbott), intubated with a 16G endotracheal tube (Becton-Dickinson), and ventilated with a rodent ventilator (Harvard Apparatus) supplying 100% supplemental oxygen. Regenerated lungs (n = 5) were removed from the bioreactor and the left lung was prepared for transplantation using a modified non-suture external cuff technique (26 (link)). In brief, the donor hilar vessels were isolated and transected to mount cuffs prepared from Venisystems Abbocaths (Hospira). Arterial and venous vessels were secured with 8-0 silk to the 18G cuff, while the bronchus was secured to a 16G cuff. A left anterior thoracotomy was performed on the non-heparinized recipient rats. The hilar vessels were dissected circumferentially and individually clamped with micro-Serrefines clamps. Donor vessels and cuffs were inserted into the recipient vessels and secured with 6-0 silk.
Blood gases were analyzed at 20 and 60 minutes after left lung reperfusion. Samples were drawn from the left pulmonary vein at the anastomosis and analyzed using an iStat Portable Analyzer with CG4+ Cartridges (Abbott). Lungs were then explanted and fixed in 10% formalin or stored in RNAlater.

Gilpin S.E., Ren X., Okamoto T., Guyette J.P., Mou H., Rajagopal J., Mathisen D.J., Vacanti J.P, & Ott H.C. (2014). Enhanced Lung Epithelial Specification of Human iPSCs on Decellularized Lung Matrix. The Annals of thoracic surgery, 98(5), 1721-1729.

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Hemodynamic Changes After Renal Denervation

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The baseline hemodynamic parameters were considered after the placement of all vascular sheaths and just before RA cannulation, and initially stable hemodynamic was noted in all animals. A Swan Ganz catheter, 6 F (B. Braun, Germany), was inserted into the distal part of the pulmonary artery (PA) through the right jugular vein under fluoroscopic control, and the pulmonary artery wedge pressure (PWP) was measured. Pressures in the PA, RV, and right atrium were measured. Arterial blood samples were taken from the abdominal aorta; venous blood samples were taken from the PA for evaluation of SatO2. Blood tests were carried out using the i-STAT portable analyzer (Abbott Laboratories, USA). Cardiac output (CO) was calculated using the Fick equation. Pulmonary vascular resistance (PVR) and systemic vascular resistance (SVR) were calculated with an established formula. Following renal artery denervation in the RDN group and balloon inflations in the SHAM group, repeated measurements were performed after a 15-minute interval.

Vakhrushev A.D., Condori Leandro H.I., Goncharova N.S., Korobchenko L.E., Mitrofanova L.B., Lebedev D.S, & Mikhaylov E.N. (2020). Extended Renal Artery Denervation Is Associated with Artery Wall Lesions and Acute Systemic and Pulmonary Hemodynamic Changes: A Sham-Controlled Experimental Study. Cardiovascular Therapeutics, 2020, 8859663.

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