Human recombinant leptin, human VEGFR-2 Quantikine ELISA Kits, and human VEGF cytokine (293-VE) was purchased from R&D Systems, Minneapolis, MN. Notch1 (sc-373891), Notch4 (sc-56594) and Jagged1 (JAG1, sc-8303) polyclonal antibodies were obtained from Santa Cruz Biotechnology, Santa Cruz, CA. DLL4 (ab7280), Notch2 (ab8926), and Notch3 (ab23426) polyclonal antibodies were purchased from Abcam, Cambridge, MA. VEGFR-2 monoclonal antibody (55B11) was purchased from Cell Signaling, Danvers, MA. Anti-mouse and anti-rabbit polyclonal antibodies conjugated to horseradish peroxidase were from Bio-Rad Laboratories, Hercules, CA. SU5416 was purchased from Sellekchem, Houston TX. Enhanced chemiluminescence (ECL)-western blot stripping buffer was from Thermo Scientific, Rockford, IL. Pegylated leptin receptor antagonist 2 (PEG-LPrA2) was prepared by us as previously described (Gonzalez and Leavis, 2003 (link)). Beta-actin (A5316) and GAPDH (glycerol aldehyde phosphate dehydrogenase; G8795) monoclonal antibodies, protease and phosphatase inhibitor cocktails 1 and 2, fetal bovine serum (FBS), DAPT [N-[N-(3,5-difluorophenacetyl)-l -alanyl]-S-phenylglycine t-butyl ester], 4’,6-diamidino-2-phenylindole] and S2188 gamma-secretase inhibitors, and other chemicals were purchased from Sigma-Aldrich, St. Louis, MO.
Beta actin a5316
Manufactured by Merck Group
Sourced in United States
Beta-actin (A5316) is a cytoskeletal protein found in all eukaryotic cells. It is commonly used as a reference gene in various molecular biology techniques, such as Western blotting and real-time PCR, to normalize gene expression data.
Automatically generated - may contain errors
Lab products found in correlation
Protease and phosphatase inhibitor cocktails 1 and 2, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Recombinant human leptin, by R&D Systems (1 mentions)
Su5416, by Selleck Chemicals (1 mentions)
Gapdh, by Merck Group (1 mentions)
Ab1511, by Merck Group (1 mentions)
Ab9708, by Merck Group (1 mentions)
Ab1555p, by Merck Group (1 mentions)
Ab1557p, by Merck Group (1 mentions)
Ab9864, by Merck Group (1 mentions)
3 protocols using beta actin a5316
1
Notch Signaling Pathway Inhibition
2
Fibroblast SHPK Protein Quantification
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The SHPK protein content of fibroblasts homogenates, untreated and treated with cyclohexamide for protein stability, from patient 1 was assessed by Western blot analysis and compared with a homogenate from control fibroblasts (untreated with cyclohexamide). Briefly, washed-cell pellets were homogenized in radioimmunoprecipitation assay (RIPA) buffer, cleared from debris by centrifugation, and 18 μg protein of each sample was resolved on a 12 % polyacrylamide gel. After blotting, SHPK protein was detected with a primary antibody against SHPK (ab69920, Abcam Inc.). Recombinant human SHPK was used to demonstrate the specificity of the SHPK antibody. Equal protein loading was indicated by beta actin (A5316, Sigma) detection.
3
Neuroreceptor Protein Detection
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Antibodies against PSD95 (AB9708), GAD65/67 (AB1511), NMDA receptor (AB9864), Glutamate receptor 1 (AB31232), Glutamate receptor 2 (AB20673), NMDA receptor 2A (AB1555p), NMDA receptor 2B (AB1557p) were obtained from Millipore (Billerica, MA, USA) and beta-actin (A5316) from Sigma Aldrich (St. Louis, MO, USA).
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