8 ohdg assay preparation reagent set
The 8-OHdG Assay Preparation Reagent Set is a product designed for the quantitative determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in biological samples. It contains the necessary reagents to prepare samples for analysis using the 8-OHdG ELISA assay.
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16 protocols using 8 ohdg assay preparation reagent set
Quantifying Mitochondrial and Nuclear DNA Damage
8-OHdG Detection Assay Protocol
Quantifying DNA Damage in L929 Cells
Quantification of Oxidative DNA Damage
Quantification of Oxidative DNA Damage
Highly Sensitive 8-OHdG Quantification
Quantification of 8-OHdG in F. nucleatum
Measuring Oxidative DNA Damage in Nrf2 Mice
used for the measurement of 8-OHdG. Renal DNA of
Nrf2−/−gpt delta mice and
Nrf2+/+gpt delta mice was extracted and
digested as described previously37 (link).
Briefly, nuclear DNA was extracted with a DNA Extractor WB Kit (Wako Pure Chemical
Industries). For further prevention of artifactual oxidation in the cell lysis step,
deferoxamine mesylate (Sigma-Aldrich) was added to the lysis buffer. The DNA was digested
to deoxynucleotides by treatment with nuclease P1 and alkaline phosphatase, using an
8-OHdG Assay Preparation Reagent Set (Wako Pure Chemical Industries). The levels of 8-OHdG
(8-OHdG/105 dG) were measured by high-performance liquid chromatography with
an electrochemical detection system (Coulochem II, ESA, Bedford, MA, USA) as previously
reported19 (link).
Quantifying Oxidative Stress in the Cerebral Cortex
DNA in the cerebral cortex (ca. 100 mg) was extracted using an extraction kit (DNA Extractor® TIS kit, Wako Pure Chemical Industries, Ltd.) (n=4/group). The obtained DNA (ca. 20 µg) was treated with nuclease P1 and alkaline phosphatase according to the manufacturer's protocol. The level of 8-oxo-deoxyguanosine (8-oxodG) in DNA was measured in triplicate using an ELISA kit (8-OHdG assay preparation reagent set, Wako Pure Chemical Industries, Ltd).
Quantifying Oxidative DNA Damage
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