Anti human cd38 clone hit2

The following fluorochrome-conjugated antibodies were used: anti-human
CD3 (clone UCHT1, 1:200), anti-human CD4 (clone RPA-T4, 1:200), anti-human CD19
(clone HIB19, 1:100), anti-human CD27 (clone O323, 1:100), anti-human CD38
(clone HIT2, 1:100), anti-human CD95 (clone DX2, 1:100), anti-human ICOS (clone
C398.4A, 1:100), anti-human CD40L (clone 24-31, 1:10), anti-human PD-1 (clone
EH12.2H7, 1:100), anti-human CXCR3 (clone G025H7, 1:200), anti-human CTLA4
(clone L3D10, 1:10), anti-human CD69 (clone FN50, 1:50) and anti-human
IL-7Rα (clone A019D5, 1:100) were all from BioLegend. Anti-human CXCR5
(clone RF8 B2, 1:200), anti-human Bcl-6 (clone K112-91, 1:20), anti-human IgD
(clone IA6-2, 1:50), anti-human Ki-67 (MOPC-21, 1:10) and anti-human CD21 (clone
B-ly4, 1:100) were from BD Biosciences and anti-human CD45RA (clone
2H4LDH11LDB9, 1:200) was from Beckman Coulter. LIVE/DEAD^®Fixable Dead Cell Stain from Invitrogen was used to gate on live cells as well
as Annexin V from BD Biosciences.

Flow cytometry was performed on BD LSR Fortessa. Transduction efficiency was measured with an APC-conjugated antibody toward NGFR (CD271; clone ME20.4, BioLegend) and Protein L for CCR, whereas for CARs it was measured in the phycoerythrin (PE) channel to detect dsRed and AffiniPure F(ab’)₂ Fragment Goat Anti-Mouse IgG (Jackson ImmunoResearch). The following antibodies were used for flow cytometry staining: Anti-Human CD3 (clone UCHT1, BD Biosciences), Anti-Human CD4 (clone L200, BD Biosciences), Anti-Mouse CD45 (clone 30-F11, BD Biosciences), Anti-Human CD8 (clone SK1, BD Biosciences), Anti-Human CD45 (clone HI30, BD Biosciences), Anti-human CD366 (TIM-3) (clone F38-2E2, BioLegend or clone F38-2E2, Thermo Fisher Scientific), Anti-human CD279 (PD-1) (clone EH12.2H7, BioLegend or clone eBioJ105, Thermo Fisher Scientific), Anti-human CD62L (clone DREG-56, BioLegend), Anti-human CD269 (BCMA) (clone 19F2, BioLegend), Anti-human CD38 (clone HIT2, BioLegend), CD45RA (clone HI100, Thermo Fisher Scientific), LAG-3 (CD223; clone 3DS223H, Thermo Fisher Scientific) (staining 1 μg per mL of sample, incubation for 30 minutes at 4°C in PBS with 1% BSA, 10% FBS and 0.1% NaN3 sodium azide). For the quantification of the CD38, the Quantibrite Phycoerythrin (PE) Fluorescence Quantitation Kit (BD) was used. Flow cytometry data analysis was performed with FCS Express 6 flow software.