Discovery chromomap dab ruo

Manufactured by Roche

The Discovery ChromoMap DAB RUO is a laboratory equipment product used for chromogenic detection in immunohistochemistry (IHC) and in situ hybridization (ISH) applications. It provides a stable, brown chromogenic reaction to visualize target analytes in biological samples.

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Lab products found in correlation

Ventana discovery ultra, by Roche (3 mentions) Discovery ultra, by Roche (2 mentions) Zen lite imaging software, by Zeiss (2 mentions) Discovery omnimap anti rabbit hrp ruo, by Roche (2 mentions) Strataquest version 5, by TissueGnostics (2 mentions) Ab16667, by Abcam (2 mentions) Discovery omni map anti rabbit hrp, by Roche (2 mentions) Axio scan z1, by Zeiss (1 mentions) Pdgfra, by Thermo Fisher Scientific (1 mentions) Discovery purple kit, by Roche (1 mentions) Ab89901, by Abcam (1 mentions) Cal ex, by Thermo Fisher Scientific (1 mentions) Ab20034, by Abcam (1 mentions) Discovery omnimap anti mouse hrp, by Roche (1 mentions)

Close spelling variants of this product

ChromoMap DAB (23 citations) Discovery ChromoMap DAB (9 citations)

5 protocols using discovery chromomap dab ruo

Automated Immunohistochemical Analysis of Ki67

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Tissue sections of 4μm were mounted on slides and immunohistochemical staining for Ki67 was performed using a fully automated immunohistochemistry (IHC) system and ready-to-use optimized reagents according to the manufacturer's recommendations (Ventana Discovery Ultra, Ventana, Arizona, USA). Primary antibody used was rabbit anti-Ki67 (AB16667, Abcam, Cambridge, UK) and secondary antibody was Discovery Omnimap anti-rabbit HRP RUO (760-4311, Roche, Rotkreuz, Switzerland). DAB kit was Discovery Chromomap DAB RUO (760-4311, Roche). After IHC procedure, slides were first evaluated for Ki67 staining quality using mouse intestine tissue as positive control. Regions containing tumour tissue were identified and marked by a pathologist and subsequently scanned in brightfield at 20x magnification using Zeiss Axio Scan.Z1 and ZEN lite imaging software (Carl Zeiss Microscopy GmbH, Jena, Germany). Digital images were then subjected to automated image analysis using StrataQuest version 5 (TissueGnostics, Vienna, Austria) for Ki67 quantification. Three different gates were set to quantify low, medium and high intensity DAB staining which corresponded to Ki67 expression levels. Results were depicted as total percentage of Ki67-positive nuclei.

Turajlic S., Xu H., Litchfield K., Rowan A., Chambers T., Lopez J.I., Nicol D., O’Brien T., Larkin J., Horswell S., Stares M., Au L., Jamal-Hanjani M., Challacombe B., Chandra A., Hazell S., Eichler-Jonsson C., Soultati A., Chowdhury S., Rudman S., Lynch J., Fernando A., Stamp G., Nye E., Jabbar F., Spain L., Lall S., Guarch R., Falzon M., Proctor I., Pickering L., Gore M., Watkins T.B., Ward S., Stewart A., DiNatale R., Becerra M.F., Reznik E., Hsieh J.J., Richmond T.A., Mayhew G.F., Hill S.M., McNally C.D., Jones C., Rosenbaum H., Stanislaw S., Burgess D.L., Alexander N.R, & Swanton C. (2018). Tracking Cancer Evolution Reveals Constrained Routes to Metastases: TRACERx Renal. Cell, 173(3), 581-594.e12.

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Dual Immunohistochemical Analysis of Stricture Regions

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Serial 4-μm-thick sections from uninflamed and inflamed regions of each block of stricture were taken to perform the following immunohistochemical analyses, as previously described^{44 (link)}. For double stains, tissue specimens were fixed in 10% formalin and embedded in paraffin and 3-μm sections were used for immunohistochemistry.
Immunohistochemistry was performed using VENTANA DISCOVERY ULTRA from Roche. This system allows for automated baking, deparaffinization and cell conditioning. Semiautomatic dual staining was performed sequentially using WT1 at a 1:25 dilution (abcam ab89901) during 60 min. An automated one drop of a prediluted secondary antibody Discovery OMNIMap anti-rabbit-HRP from Roche (760–4310) was used and the signal was obtained using Discovery ChromoMap DAB RUO from Roche (760–2513) (brown signal). PDGFRA (Thermofisher TA804956) was used at a 1:50 dilution during 60 min and after this, secondary antibody (Discovery OMNIMap anti-mouse-NP from Roche (760–4816)) positive signal was obtained using Discovery Purple Kit (760–229)(purple signal). Tissues were counterstained with haematoxylin to visualize the nuclei.

Nayar S., Morrison J.K., Giri M., Gettler K., Chuang L.S., Walker L.A., Ko H.M., Kenigsberg E., Kugathasan S., Merad M., Chu J, & Cho J.H. (2021). A myeloid–stromal niche and gp130 rescue in NOD2-driven Crohn’s disease. Nature, 593(7858), 275-281.

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Histopathological Evaluation of Arthritis in Mice

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At the end of the arthritis observation period mice were euthanized, the paws fixed in 10% formaldehyde and then decalcified with a solution containing hydrochloric acid and 0.1 M EDTA (Cal-Ex; Fisher Scientific, Fairlawn, NJ). Tissues were embedded in paraffin, and slides prepared and stained with hematoxylin-eosin. The slides were evaluated in a blinded manner with a combined scoring system that included the following parameters: synovial inflammation, synovial hyperplasia, cartilage, and bone erosions (Supplemental Table S1).28 (link), 29 (link), 30 (link), 31 (link)
Small intestine and colon were collected and “Swiss-rolled” for histology.³² Immunohistochemistry (IHC) was done with the Ventana Discovery Ultra (Roche, Switzerland). Mouse anti-Foxp3 monoclonal antibody (ab20034, Abcam, Cambridge, UK) was used at a 1:6000 dilution and incubated for 30 min. Discovery OmniMap anti-mouse-HRP (Roche 760-4310) was used as secondary antibody and the signal acquired using Discovery ChromoMap DAB RUO (Roche 760-2513). Tissues were then stained with Hematoxylin to visualize the nuclei.

Laragione T., Harris C., Azizgolshani N., Beeton C., Bongers G, & Gulko P.S. (2023). Magnesium increases numbers of Foxp3+ Treg cells and reduces arthritis severity and joint damage in an IL-10-dependent manner mediated by the intestinal microbiome. eBioMedicine, 92, 104603.

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Multiplex Immunohistochemistry for Tissue Analysis

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Slides with 5um sections from paraffin embedded tissues from autopsies were stained with CD3 (LN10) and GFAP (GA5) pre-diluted Bond reagents from Leica Microsystems, HEIR (Heat Induced Epitope Retrieval) for 20 minutes with ER2 (Bond Epitope Retrieval Solution 2), MSMC DAB detection, counterstained per established staining protocol on the automated Leica Bond Biosystems III platform (USA, Buffalo Grove, IL). Immunohistochemistry for BCMA was performed using VENTANA DISCOVERY ULTRA from Roche. This system allows for automated baking, deparaffinization and cell conditioning. Semiautomatic staining was performed using BCMA antibody (cat#B0807 fromUSBiological) at 1:10 dilution during 60 min. As secondary antibody Discovery OMNIMap anti-rabbit-HRP from Roche (760-4310) was used and the signal was obtained using Discovery ChromoMap DAB RUO from Roche (760-2513) (brown signal) Tissues were counterstained with Hematoxylin (in blue).

Van Oekelen O., Aleman A., Upadhyaya B., Schnakenberg S., Madduri D., Gavane S., Teruya-Feldstein J., Crary J.F., Fowkes M.E., Stacy C.B., Kim-Schulze S., Rahman A., Laganà A., Brody J.D., Merad M., Jagannath S, & Parekh S. (2021). Neurocognitive and hypokinetic movement disorder with features of parkinsonism following BCMA-targeting CAR-T cell therapy. Nature medicine, 27(12), 2099-2103.

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Automated Ki67 Quantification in Tumor Tissue

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Tissue sections of 4μm were mounted on slides and immunohistochemical staining for Ki67 was performed using a fully automated immunohistochemistry (IHC) system and ready-to-use optimized reagents according to the manufacturer's recommendations (Ventana Discovery Ultra, Ventana, Arizona, USA). Primary antibody used was rabbit anti-Ki67 (AB16667, Abcam, Cambridge, UK) and secondary antibody was Discovery Omnimap anti-rabbit HRP RUO (760-4311, Roche, Rotkreuz, Switzerland). DAB kit was Discovery Chromomap DAB RUO (760-4311, Roche). After IHC procedure, slides were first evaluated for Ki67 staining quality using mouse intestine tissue as positive control. Regions containing tumor tissue were identified and marked by a pathologist and subsequently scanned in brightfield at 20x magnification using Zeiss Axio Scan.Z1 and ZEN lite imaging software (Carl Zeiss Microscopy GmbH, Jena, Germany). Digital images were then subjected to automated image analysis using StrataQuest version 5 (TissueGnostics, Vienna, Austria) for Ki67 quantification. Three different gates were set to quantify low, medium and high intensity DAB staining which corresponded to Ki67 expression levels. Results were depicted as total percentage of Ki67-positive nuclei.

Turajlic S., Xu H., Litchfield K., Rowan A., Horswell S., Chambers T., O’Brien T., Lopez J.I., Watkins T.B., Nicol D., Stares M., Challacombe B., Hazell S., Chandra A., Mitchell T.J., Au L., Eichler-Jonsson C., Jabbar F., Soultati A., Chowdhury S., Rudman S., Lynch J., Fernando A., Stamp G., Nye E., Stewart A., Xing W., Smith J.C., Escudero M., Huffman A., Matthews N., Elgar G., Phillimore B., Costa M., Begum S., Ward S., Salm M., Boeing S., Fisher R., Spain L., Navas C., Grönroos E., Hobor S., Sharma S., Aurangzeb I., Lall S., Polson A., Varia M., Horsfield C., Fotiadis N., Pickering L., Schwarz R.F., Silva B., Herrero J., Luscombe N.M., Jamal-Hanjani M., Rosenthal R., Birkbak N.J., Wilson G.A., Pipek O., Ribli D., Krzystanek M., Csabai I., Szallasi Z., Gore M., McGranahan N., Van Loo P., Campbell P., Larkin J, & Swanton C. (2018). Deterministic Evolutionary Trajectories Influence Primary Tumor Growth: TRACERx Renal. Cell, 173(3), 595-610.e11.

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