The concentrations of inflammatory factors IFN-γ, IL-1β, and IL-6 in nasal mucosal tissues were assessed using ELISA assays. A total of 1 mL 0.9% NaCl was added to each 0.1 g sample, and all samples were homogenized at 1000 rpm for 5 min and centrifuged at 1500 × g for 10 min at 4°C to collect the supernatant. Concentrations of inflammatory factors IFN-γ (DIF50C, R&D Systems, Minneapolis, MN, USA), IL-1β (DLB50, R&D Systems), IL-6 (D6050, R&D Systems), IL-4 (PI618, Beyotime), and IL-13 (ab47353, Abcam, Cambridge, MA, USA) in nasal mucosal tissues of patients with CRSsNP and controls were measured using ELISA kits.
The concentrations of inflammatory factors IFN-γ (MIF00, R&D Systems), IL-1β (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems), IL-4 (PI612, Beyotime), and IL-13 (ab219634, Abcam) in nasal mucosal tissues of mice in the CRSsNP and control groups were assessed using ELISA kits as well. All operations were performed strictly in accordance with the ELISA kit instructions.
The concentrations of inflammatory factors IFN-γ (MIF00, R&D Systems), IL-1β (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems), IL-4 (PI612, Beyotime), and IL-13 (ab219634, Abcam) in nasal mucosal tissues of mice in the CRSsNP and control groups were assessed using ELISA kits as well. All operations were performed strictly in accordance with the ELISA kit instructions.