Systolic blood pressure was measured in conscious male and female mice using the tail-cuff method (Kent Scientific Inc.). Briefly, animals were placed in a mouse restrainer (RTBP007, Kent Scientific Inc.), and a mouse occlusion cuff (RTBP050, Kent Scientific Inc.) and mouse plethysmographic cuff (XBP051, Kent Scientific Inc.) were applied to the tail of the mouse. Mice were trained for 1 week to become accustomed to the new handling and environment. Systolic blood pressure measurements were taken using the XBP1000 apparatus (Kent Scientific Inc.) connected to a data acquisition system. Five consecutive measurements were performed per mouse, per time point, and the values were averaged. Measurements were done at the same time of the day at the indicated time points.
Rtbp050
Manufactured by Kent Scientific
The RTBP050 is a recirculating thermoelectric bath that provides temperature control from -50°C to 100°C. It circulates temperature-controlled fluid to maintain a stable temperature for various applications. The RTBP050 features a compact design and digital temperature display.
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2 protocols using rtbp050
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Tail-cuff blood pressure in mice
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Tail-cuff blood pressure in mice
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Systolic blood pressure was measured in conscious male and female mice using the tail-cuff method (Kent Scientific Inc.). Briefly, animals were placed in a mouse restrainer (RTBP007, Kent Scientific Inc.), and a mouse occlusion cuff (RTBP050, Kent Scientific Inc.) and mouse plethysmographic cuff (XBP051, Kent Scientific Inc.) were applied to the tail of the mouse. Mice were trained for 1 week to become accustomed to the new handling and environment. Systolic blood pressure measurements were taken using the XBP1000 apparatus (Kent Scientific Inc.) connected to a data acquisition system. Five consecutive measurements were performed per mouse, per time point, and the values were averaged. Measurements were done at the same time of the day at the indicated time points.
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