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Screen printed carbon electrodes spces

Manufactured by Metrohm
Sourced in Spain

Screen-printed carbon electrodes (SPCEs) are a type of electrochemical sensor that consists of a carbon-based working electrode, a reference electrode, and a counter electrode, all printed on a substrate. SPCEs are designed to provide a versatile and cost-effective platform for various electrochemical measurements and applications.

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3 protocols using screen printed carbon electrodes spces

1

Electrochemical Immunosensor for NSE Detection

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Graphite powder was obtained from Alfa Aesar (USA). Melamine, dopamine hydrochloride, uric acid, potassium hexacyanoferrate(III), and all other reagents were purchased from Sigma-Aldrich (USA). Screen-printed carbon electrodes (SPCEs) were acquired from DropSens (Spain). Anti-neuron specific enolase (Anti-NSE) monoclonal antibodies and NSE antigen were purchased from Abcam (USA). Phosphate buffered saline (PBS, 0.1 M, pH 7.4) was prepared by mixing stock solutions of NaH2PO4 and Na2HPO4 and was used as the supporting electrolyte. All aqueous solutions were prepared with deionized (DI) water.
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2

Electrochemical Sensor Modification with AuNPs

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Screen-printed
carbon electrodes (SPCEs) were purchased from DropSens, Spain. The
SPE platform used was DRP-100 (named SPCE), constituted of a 4 mm
diameter carbon working electrode, a silver pseudo-reference electrode,
and a carbon auxiliary electrode. To modify the bare SPCE, a certain
volume of AuNPs at a concentration of 5 mg/mL was directly drop cast
onto the surface of the working electrode and left at room temperature
to dry until further use.
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3

Voltammetric Measurements using SPCEs

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Screen printed carbon electrodes (SPCEs) were purchased from Dropsens (Asturias, Spain), and consisted of the working electrode (carbon), reference electrode (Ag), and auxiliary electrode (carbon). Voltammetric measurements were obtained using the Autolab (Ecochemie, Utrecht, The Netherlands) potentiostat incorporated with a general-purpose electrochemical system (NOVA 1.11).
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