All chemicals and reagents used in the experiments were of per analysis and HPLC grade. Folin–Ciocalteu reagent, potassium chloride, sodium hydrogencarbonate, potassium hydrogenphosphate, magnesium chloride hexahydrate, sodium chloride, ammonium carbonate, sodium hydroxide, and petroleter were supplied from Kemika d.d. (Zagreb, Croatia). Anhydrous sodium carbonate and sodium nitrite were acquired from Gram-Mol d.o.o. (Zagreb, Croatia). Sodium molybdate (>98%) was supplied from abcr Gmbh (Karlsruhe, Germany). Methanol, acetonitrile, concentrated hydrochloric acid (37%, v/v), sodium hydroxide, and formic acid were purchased from Carlo Erba Reagents (Val de Reuil, France). Secondary analytical standards, i.e., echinacoside (>98%) and verbascoside (>98%), were obtained from Biosynth s.r.o. (Bratislava, Slovakia), whereas caffeine was supplied from Fluka (Taufkirschen, Germany). Theobromine (min. 99%), human salivary α–amylase XII-A (Enzyme Commission number: 3.2.1.1.; 1149 U mg−1 protein), pepsin from porcine gastric mucosa (474 U mg−1 protein), pancreatin from porcine pancreas (4×USP), and bile bovine salts (unfractionated) were provided from Sigma Aldrich (St. Louis, MO, USA).
Sodium chloride (nacl)
Manufactured by Kemika
Sourced in Croatia, United States
Sodium chloride is a chemical compound with the formula NaCl. It is a white crystalline solid that is commonly known as table salt or halite. Sodium chloride is a commonly used laboratory reagent and is used in various scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Kemika (4 mentions)
Potassium chloride (kcl), by Kemika (3 mentions)
Ethanol, by Kemika (2 mentions)
Myrtenol standard, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Carlo Erba (1 mentions)
Pepsin from porcine gastric mucosa, by Merck Group (1 mentions)
Formic acid, by Carlo Erba (1 mentions)
Sodium hydrogencarbonate, by Kemika (1 mentions)
Pancreatin from porcine pancreas 4 usp, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Kemika (1 mentions)
Methanol, by Carlo Erba (1 mentions)
Acetonitrile, by Carlo Erba (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Molecular biology grade dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Normal melting point agarose, by Merck Group (1 mentions)
Tris buffer, by Merck Group (1 mentions)
11 protocols using sodium chloride (nacl)
1
Phytochemical Analysis of Botanical Extracts
2
Cytotoxicity Assay Protocol
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Acetonitrile, ethyl acetate, formic acid and methanol were purchased from Kefo (Sisak, Croatia); Czapek Yeast Agar (CYA) was purchased from Oxoid (Hampshire, UK). Medium RPMI 1640, fetal bovine serum (FBS), phosphate buffered saline (PBS; without Ca2+ and Mg2+), trypsin-EDTA, antibiotics penicillin and streptomycin were from Lonza (Basel, Switzerland). The MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], acridine orange, ethidium bromide, normal melting point agarose (NMP), low melting point agarose (LMP), phorbol 12-myristate 13-acetate (PMA), Triton X-100 and Tris buffer were purchased from Sigma-Aldrich (Deisenhofen, Germany) and molecular biology grade dimethyl sulfoxide (DMSO), used for dissolving the extracts and for the control treatment, was purchased from Sigma-Aldrich (Deisenhofen, Germany); technical grade DMSO, used for dissolving the formazane, ethanol, sodium chloride, disodium-EDTA and sodium hydroxide were from Kemika (Zagreb, Croatia).
3
Sodium Chloride Solution Preparation
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Sodium chloride was purchased from Kemika (Zagreb, Croatia). Petroleum ether and ethanol were bought from Carlo Erba (Val de Reuil Cedex, France). The Millipore apparatus was used to obtain deionised water for the preparation of Sodium chloride solution.
4
Purification and Characterization of Apo-Transferrin
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Native human apo-transferrin (Biorbyt, UK, cat. no. orb80927), sodium acetate trihydrate (Kemika, Croatia), calcium chloride (Lach-Ner, Croatia), sodium chloride (Kemika, Croatia), neuraminidase (GlycoCleave® Neuraminidase Kit, GALAB Technologies, Germany, cat. no. 132011), pISep Buffer Kit (CryoBioPhysica, USA, cat. no. 20055), hydrogen chloride (Carlo Erba Reagents, Italy, 37 % solution), sodium hydroxide (Kemika, Croatia, pellets 2–5 mm), sodium phosphate (Kemika, Croatia), guanidine hydrochloride (PanReac AppliChem, USA), MES (2-(N-morpholino)ethanesulfonic acid, Sigma Aldrich, USA) and potassium chloride (Alkaloid, North Macedonia) were used without further purification. Water used for experiments was double distilled in an all-glass apparatus. All experiments except the enzymatic desialylation were performed at room temperature.
5
Trace Element Analysis Protocol
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In order to conduct this study, myrtenol standard was purchased from Sigma-Aldrich (St. Lois, MO, USA) and sodium chloride was obtained from Kemika (Zagreb, Croatia). Standards of Se, K, Ca, Mn, Fe, Cu, Zn, Br, Rb, Sr and Pb (1 g/L) were purchased from TraceCERT, Fluka Analytical (St. Gallen, Switzerland).
6
Trace Element Analysis Protocol
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In this study, the following reagents and standards were used: myrtenol (Sigma-Aldrich, St. Lois, MO, USA), sodium chloride (Kemika, Zagreb, Croatia), and element standards of Se, K, Ca, Mn, Fe, Cu, Zn, Br, Rb, Sr and Pb (TraceCERT, Fluka Analytical, St. Gallen, Switzerland).
7
Aroma Compound Analysis Protocol
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In this study, the following chemicals were used to determine aroma compounds: myrtenol standard (Sigma-Aldrich, St. Lois, MO, USA) and sodium chloride (Kemika, Zagreb, Croatia).
8
Analytical Reagents for Research Protocols
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All reagents and solvents used for the analyses were of analytical grade. Pepsin was obtained from Sigma Aldrich GmbH (Taufkir-chen, Germany), salts (NaCl, KCl, NaH2PO4, Na2SO4, NaHCO3, CO(NH2)2, CaCl2), ethanol, and hydrochloride acid were obtained from Kemika (Zagreb, Croatia). DPPH was obtained from Merck KgaA (Darmstadt, Germany).
9
gp96 Binding Assay in Decidual Macrophages
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The binding of gp96 for CD91 AND tlr4 was assessed in freshly isolated DMC from normal first-trimester early pregnancy. DMC (2.5 × 105 cells/sample) were untreated or treated with gp96 (Sigma Aldrich Chemie, Steincheim, Germany) at a concentration of 0.125 µg/mL, 0.25 µg/mL, 0.5 µg/mL, and 1 µg/mL for 30 min on ice. Then fluorescein isothiocyanate (FITC), either conjugated anti-CD91 mAb (IgG2b) or TLR4 (IgG1) mAb diluted 1:100 in fluorescence-activated cells sorter (FACS) buffer [NaCl (8.12 g/L), KH2PO4 (0.26 g/L), Na2HPO4 (2.35 g/L), KCl (0.28 g/L), all from Kemi-ka, Zagreb, Croatia; Na2EDTA (0.36 g/L), Fluka, Buchs, Switzerland; NaN3 (0.1 g/L)] was added into the samples for 30 min. FITC conjugated mouse IgG1 or FITC conjugated mouse IgG2b, respectively, were used as isotype controls. After washing in FACS buffer, CD1a was labeled using PE-conjugated anti-CD1a mAb added, respectively (30 min. at +4 °C).
10
Indole-3-Acetic Acid Biosynthesis Pathway
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Murashige and Skoog (MS) powder medium containing vitamins, plant agar, and 2-(N-morpholino)ethanesulfonic acid (MES) monohydrate were obtained from Duchefa, Haarlem, The Netherlands. Sucrose, NaCl, mannitol were purchased from Kemika, Zagreb, Croatia, while Izosan G was purchased from Pliva, Zagreb, Croatia. Propidium iodide (PI) was obtained from Sigma-Aldrich, St. Louis, MO, USA. Standards for IAA and its precursors anthranilate (ANT), indole-3-acetaldehyde (IAAld), IAM, indole-3-acetonitrile (IAN), IPyA, tryptamine (TRA), and tryptophan (TRP) were purchased from Sigma-Aldrich, and standards for IAOx and 2-oxoindole-3-Acetic acid (oxIAA) from OlChemIm Ltd, Olomouc, Czech Republic. Acetic acid was purchased from Merck (Darmstadt, Germany), diethyldithiocarbamic acid sodium salt and cysteamine hydrochloride from Sigma-Aldrich, St. Louis, MO, USA, and HPLC gradient grade solvents from J.T. Baker—Fisher Scientific, Hampton, NH, USA. All other chemicals were from Carl Roth, Karlsruhe, Germany and Sigma-Aldrich.
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