Hplc gradient grade water

Chemicals used for the method development included the HPLC gradient grade water, from Sigma-Aldrich (99.8% pure), acetonitrile (98.9% pure), the 1-hydroxypyrene biomarker (1-OHPyr, linear formula (LF) = C 16 H 10 O, molecular weight (MW) = 218.25, 98% pure), that was purchased from sigma-Aldrich. All the glassware (Pyrex) was thoroughly washed ning the importance of the UV-visible detections for this purpose since the UV-visible detector also gives good results, although it is not as sensitive as the florescent detector. In order to develop a new method based on the UV-visible detector system, we used 1-OHPyr concentrations up to the nm/l level, with an average elusion time less than 10 min i.e., 6.37 min (Table 1 and2). We have demonstrated that quantification of the 1-OHPyr using the UV-visible detector can also provide good results. The detector response provided a recovery percentage rate of more than 95% (Table 1 and2). Overall recovery remained up to 108%. The linearity of the method ranged between R 2 = 0.979 and 0.989. The overall percentage of the standard recovery is mentioned in the Table 1 and2.