N succinyl ala ala ala p nitroanilide sana

Trizma Base, N-Succinyl-Ala-Ala-Ala-p-nitroanilide/SANA, porcine pancreas elastase, and gallic acid were purchased from Sigma Aldrich (Germany). Hydrochloric acid, dimethyl sulfoxide, ethanol and methanol, Folin–Ciocalteu reagent, and sodium carbonate were purchased from Merck (Germany). Glycerine, propylene glycol, and edetate sodium were purchased from Amresco Chemicals (China). Hydroxyethyl cellulose was purchased from Fracxchange (USA). Propylparaben and methylparaben were purchased from Gujarat Organic (India). Distilled water was purchased from Brataco Chemika (Indonesia).

Ascorbic acid, collagenase from Clostridium histolyticum, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH^•), 2,2′-azino-bis-(3-ethyl-benzothiazole-6-sulfonic acid) (ABTS^●+), elastase from a porcine pancreas, (-)-epigallocatechin gallate (EGCG), hyaluronidase from the bovine tests, hyaluronic acid sodium salt from a rooster comb, Folin–Ciocalteu reagent, ethylenediaminetetraacetic acid, disodium dihydrate (Na₂EDTA*2H₂O), Tricine (≥99%; titration), N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), and N-Succinyl-Ala-Ala-Ala-p-nitroanilide (SANA) were obtained from Sigma-Aldrich (Steinheim, Germany). Phosphate-buffered saline (PBS) was purchased from Gibco (Carlsbad, CA, USA). Reference substances were supplied by ChromaDex (Irvine, CA, USA), while acetonitrile, formic acid, and water for LC analysis were supplied by Merck (Darmstadt, Germany). All other chemicals were of analytical grade and were obtained from the Polish Chemical Reagent Company (POCH, Gliwice, Poland).