The anti-proteases cocktail and Leptomycin B and the rest of the reagents were purchased from Sigma-Aldrich.
Anti proteases cocktail
Manufactured by Merck Group
The Anti-proteases cocktail is a laboratory product designed to inhibit the activity of proteases, which are enzymes that break down proteins. It is a mixture of various protease inhibitors that can be used to preserve the integrity of protein samples during experiments or analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti proteases cocktail
1
Protease Inhibitor Cocktail Protocol
2
Multiplex Cytokine Profiling of Bovine Mammary Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mammary tissue was weighed to normalization before grounding using ceramic beads (Bio-Rad) in a mixture containing Triton X-100 and a anti-proteases cocktail (Sigma). Plasma, milk, and mammary tissue concentrations for 15 cytokines (IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-6, IL-17a, IFNγ, CCL-2, CCL-3, CCL-4, CXCL8, CXCL10, and TNFα) were determined using a Custom bovine cytokines MilliPlex xMAP assay (Merck Millipore). Data were recorded on a MAGPIX flow cytometer using Xponent software (Luminex).
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