N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy-sulfosuccinimide sodium salt (NHS); 3-(N-Morpholino)propanesulfonic acid sodium salt (MOPS); tetraethoxyorthosilicate (TEOS); and 3-(aminopropyl)triethoxysilane (APTS) were purchased from Sigma-Aldrich (St Quentin Fallavier, France). Citric acid was purchased from Merck (Nogent sur Marne, France). 2-[Methoxy(polyethyleneoxy)propyl]-trimethoxysilane (PEOS), containing 3-6 ethylene oxide groups, was purchased from Gelest (Morrisville, PA, USA). 30% H2O2, HCl and NaOH solutions were obtained from VWR (Strasbourg, France). The oligonucleotides were purchased from Integrated DNA Technology (Leuven, Belgium). The probe was 21-basis long and was modified with a carboxy-end for conjugation to silica coated magnetic nanoparticles. The target was an unmodified DNA sequence of 20 bases as written in Table 1 mimicking the fragment of interest, miRNA-122, for the diagnosis of liver cells in case of injury (hepatitis, alcoholism, obesity).
3 aminopropyl triethoxysilane apts
Manufactured by Merck Group
Sourced in France, China, United States
3-(aminopropyl)triethoxysilane (APTS) is a silane coupling agent that contains an amino group and three ethoxy groups. It is commonly used as a surface modifier and adhesion promoter in various applications, including the preparation of functionalized surfaces and the manufacture of composite materials.
Automatically generated - may contain errors
Lab products found in correlation
2 methoxy polyethyleneoxy propyl trimethoxysilane peos, by Gelest (1 mentions)
N hydroxysulfosuccinimide sodium salt, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Oligonucleotide, by Integrated DNA Technologies (1 mentions)
3 trihydroxysilyl propyl methylphosphonate thpmp, by Merck Group (1 mentions)
Cell incubator, by Thermo Fisher Scientific (1 mentions)
Tetraethoxysilane teos, by Sinopharm (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
4 protocols using 3 aminopropyl triethoxysilane apts
1
Silica-Coated Magnetic Nanoparticles for miRNA-122 Detection
2
Magnetic Bead Functionalization Protocol
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Paramagnetic, 10 μm diameter glass beads (Millipore Sigma) were incubated at 23°C overnight in a solution of 20 mM HEPES (pH 7.4) and 100 mM NaCl. Equal molar amounts of (3-aminopropyl)triethoxysilane (APTS; Sigma–Aldrich) and 3-(trihydroxysilyl)propylmethylphosphonate (THPMP; Sigma–Aldrich) were subsequently added to a suspension of hydrolyzed NHS ester-activated beads in deionized water (Bagwe et al., 2006 (link); Soto-Cantu et al., 2012 (link)). Beads were derivatized at a density of 5 × 106/mL, and the organosilane reagents were included at 1000-fold excess of what would be required to coat the bead surface (based on four silane molecules per nm2; Soto-Cantu et al., 2012 (link)). The reaction was allowed to proceed for 5 hr at 50°C with shaking and then terminated with three cycles of washing in water (using a magnet to recover the beads after each wash cycle). Beads were stored at 4°C in a sterile solution of 20 mM HEPES (pH 7.2) and 100 mM NaCl at 4°C.
3
Synthesis of Carboxylated Mesoporous Silica
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CMSs (sized ~600 nm in diameter) were synthesized by the hydrothermal method. Glucose (C6H12O6), melamine (MEL), sodium dodecyl benzene sulfonate (SDBS), N,N-two methyl formamide (DMF), absolute ethanol, phosphoric acid (H3PO4), N,N’-Dicyclohexylcarbodiimide (DCC), and benzene were all analytical reagent (AR) grade and obtained from Beijing Chemical works, Beijing, China. 3-Aminopropyltriethoxysilane (APTS) was purchased from Sigma-Aldrich (Shanghai, China) and PET pellets (SD500) with a viscosity index of 0.68 dL/g from Sinopec Yizheng, Jiangsu, China.
4
Folic Acid-Functionalized Nanomaterials for Enhanced HepG2 Cell Targeting
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HepG2 cells were obtained from the cell bank of the Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cell culture reagents were purchased from Gibco, Carlsbad, CA, USA. Chlorauric acid (HAuCl4·3H2O), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), folic acid (FA) and (3-aminopropyl)triethoxysilane (APTS) were purchased from Sigma (St. Louis, MO, USA); cetyltrimethylammonium bromide (CTAB), silver nitrate (AgNO3) and sodium borohydride (NaBH4) were from Aladdin (Shanghai, China); tetraethoxysilane (TEOS) and ascorbic acid were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 2,5-Diphenyltetrazolium bromide (MTT) and cell apoptosis reagents were purchased from BestBio (Shanghai, China). Polymerase chain reaction (PCR)-related reagents and cell incubators were purchased from Thermo Fisher Scientific, Inc. (Rockford, IL, USA). Anti-Bax (ab77566), anti-Bcl-2 (15071s) and anti-Ki-67 (PB0065) antibodies were purchased from Abcam (Cambridge, UK), Cell Signaling Technology (Danvers, MA, USA) and Boster Biological Technology, Ltd. (Wuhan, China), respectively.
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