Anti ndrg1 antibody wh0010397m3

The Pathscan Sampler Kit (#7851) and Apoptosis Antibody Sampler Kit (#9930) including anti-cleaved PARP, anti-cleaved caspase-3, anti-cleaved caspase-8, and anti-cleaved caspase-9 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). The In Situ Cell Death Detection Kit (11684795910) was obtained from Roche (Indianapolis, IN, USA). Anti-P53 (#2524), anti-Bcl-2 (#15071) antibodies were also obtained from Cell Signaling Technology. Anti-NDRG1 antibody (WH0010397M3) was from Sigma Aldrich (St. Louis, MO, USA). Anti-PKCα (#2056) antibody was obtained from Cell Signaling Technology. Anti-PKCβ (2099) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Oxaliplatin (3422) was provided by Sanofi (Paris, France). The PKC inhibitor GO6976 (S7119) was obtained from Selleck (Houston, TX, USA).

For routine western blot, cells were lysed in cell lysis solution (Thermo Scientific, MA, USA). Total protein (50μg) was resolved by 12.5 % SDS-PAGE and transferred onto 0.22-lm polyvinylidene fluoride membranes (Millipore, MA, USA). The membranes were probed with appropriate antibodies overnight at 4 °C. After washing for three times, the membrane was incubated in HRP-labeled secondary antibody for 2 hours at room temperature. Protein level was normalized to GAPDH. Antibodies used in our study were listed as follows: Apoptosis Antibody Sampler Kit (#9930) including anti-PARP, anti-cleaved-caspase-3, and anti-cleaved-caspase-8 antibodies were purchased from Cell Signaling Technology. Anti-Bcl-2 (#15071), anti-Puma (#12450), anti-Bax (#5023), anti-DR4 (#42533), anti-DR5 (#8074), antibodies were also obtained from Cell Signaling Technology. Anti-NDRG1 antibody (WH0010397M3) was from Sigma Aldrich (St. Louis, MO, USA).