The anti-human mAbs have been used: CD3 PE (from Beckman Coulter), CD3 PErCP, CD11b PE, CD14 APC, CD56 PE, CD56 APC, CD73 PE, CD107a FITC, Granzyme A FITC, Perforin FITC, pJNK PE (from BD Biosciences), HLA-DR FITC (Dako), CD44 FITC, CD105 FITC, Granzyme B PE, Granzyme K Alexa647 (Immunotools), CD90 APC, COX2 FITC (Cayman chemicals). Each flow cytometric analysis was controlled with isotype-matched mAbs. All flow cytometry-based experiments were performed on FACS Calibur using Cell-Quest Pro Software. Offline data analysis was done on Summit 5.1 software.
Cd44 fitc
Manufactured by Agilent Technologies
Sourced in Denmark
The CD44 FITC is a lab equipment product that is used to detect and quantify the expression of the CD44 cell surface receptor. It is a fluorescently labeled antibody that specifically binds to the CD44 protein. The FITC (Fluorescein Isothiocyanate) label allows for the visualization and measurement of CD44 expression levels using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b pe, by BD (1 mentions)
Cd56 apc, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
Cd56 pe, by BD (1 mentions)
Cd14 apc, by BD (1 mentions)
Cd107a fitc, by BD (1 mentions)
Granzyme a fitc, by BD (1 mentions)
Perforin fitc, by BD (1 mentions)
Cd3 percp, by Beckman Coulter (1 mentions)
Cd3 pe, by Beckman Coulter (1 mentions)
Cd44 fitc, by Thermo Fisher Scientific (1 mentions)
Cd34 pe, by Beckman Coulter (1 mentions)
Cd61 pc7, by Beckman Coulter (1 mentions)
Navios cytometer, by Beckman Coulter (1 mentions)
Cd41 fitc, by Thermo Fisher Scientific (1 mentions)
Cd45 kro, by Beckman Coulter (1 mentions)
Cd34 pe, by Agilent Technologies (1 mentions)
Cd73 pe, by Agilent Technologies (1 mentions)
Cd45 fitc, by Agilent Technologies (1 mentions)
3 protocols using cd44 fitc
1
Multiparametric Flow Cytometry Analysis
2
Immunophenotyping of Leukemia Cells
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A variety of Ph+ leukemia cells and K562 cells carrying either Snail or Twist1 were re-suspended in PBS plus 0.5% BSA to a density of 1 × 107 cell/ml. Antibodies against CD34 PE (#IM1459u, Beckman Coulter), CD117 PCy7 (#IM3698, Beckman Coulter), CD45 KRO (#A96416, Beckman Coulter), CD61 PC7 (#IM3716, Beckman Coulter), CD41 FITC (#9011-0419, eBioscience), CD44 FITC (#F7083, DAKO, Denmark), CD44 FITC (#9011-0441, eBioscience) and CD184 APC (#FAB173A, R&D Systems, Minneapolis, MN, USA ) were titrated for use or added at the volumes suggested by the manufacturer and the mixture was incubated on ice for 40 min. After washing with PBS and 0.5% BSA, the labeled cells were analyzed on a Beckman Coulter Navios cytometer.
3
Phenotyping of Cultured hAM-MSCs
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The expression of MSCs-specific surface markers was studied on the cultured hAM-MSCs after passage 3. The cells were separately stained with a set of fluorochrome-conjugated antibodies [CD29-FITC, CD105-FITC, CD44-FITC, CD73-PE, CD34-PE, CD166-PE and CD45-FITC (all from Dako, Produktionsvej, Denmark)] for 45 min at 4 °C. After the washing step, the cells were analyzed by flow cytometry technique (Partec 3, USA).
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