Varian unity as500 spectrometer

Whole, commercially-purchased reagents were used directly, without purification. Mass spectrometry (MS) data were collected from an Expression CMS (Advion, Ithaca, NY, USA). CDCl₃ and dimethyl sulfoxide (DMSO)-d₆ were used for nuclear magnetic resonance (NMR) and chemical transformation unit and are expressed as parts per million (ppm), with an altering residual solvent or deuterated peaks (δ_H 7.24 and δ_C 77.0 for CDCl₃, δ_H 2.50 and δ_C 39.7 for DMSO-d₆). For the detection of NMR data, the Unity INOVA 400 spectrometer or a Varian Unity AS500 spectrometer (Agilent Technologies, Santa Clara, CA, USA) was used. The coupling constant is expressed in Hz. All of the reactions were controlled by thin-layer chromatography atmospheric nitrogen using Merck precoated 60F₂₅₄ plates (Billerica, MA, USA).

All the reagents were achieved commercially and used without further purification. High-resolution MS (HRMS) data were obtained on a 6530 Accurate Mass quadrupole time-of-flight liquid-chromatograph mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). ¹H and ¹³C nuclear magnetic resonance (NMR) spectral were recorded in CD₃OD (δ_H 3.30 and δ_C 49.0), CDCl₃ (δ_H 7.24 and δ_C 77.0), and DMSO-d₆ (δ_H 2.50 and δ_C 39.7) on a Varian Unity INOVA 400 spectrometer or a Varian Unity AS500 spectrometer (Agilent Technologies).