Cd45 pc7

Animal care and ABMC studies were carried out according to guidelines of the animal care committee of both Rutgers University and the Faculty of Medicine at Cairo University. Twenty-two adult male mixed-breed dogs (3 to 4 years old) were obtained from local vendors and housed at the vivarium of the Faculty of Veterinary Medicine at Cairo University. Canine ABMCs were isolated from the femurs of six adult dogs for in vitro studies, as we recently described for human ABMCs. Canine ABMCs were subjected to flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA) after staining with 100 μl of labeled antibodies (appropriately diluted to previously determined titration points) against the following cell surface markers: cluster of differentiation 13-phyco-erythrin (PE) cyanine 7 (cy7) (CD13-PC7), CD29-PC7, CD34-PE, CD44-fluorescein isothiocyanate (FITC), CD45-PC7, CD73-PE, CD90-PE, CD105-PE, CD166-PE, CD271-PE, and c-kit-PE (all from BD Biosciences). Dead cells were excluded by labeling with 1 μg/ml of 7-aminoac-tinomycin D (7-AAD; Invitrogen, Carlsbad, CA, USA). Mesenchymal induction into osteogenic, adipogenic, and chondrogenic lineages was performed as previously described (18 (link),34 (link)). Green fluorescent protein (GFP) labeling and neural induction were performed as previously described (2 ,9 (link)) with modifications described below.