Protein lysates were prepared in RIPA lysis buffer, and separated on 10% SDS–polyacrylamide gel electrophoresis gels by electrophoresis. Subsequently, the lysates were transferred to nitrocellulose membranes and probed with the following antibodies and concentrations: HIF-2α 1:1,000 (Novus #NB100-122), HIF-1α 1:1,000 (Cayman Chemicals #1006421), caspase-3 1:1,000 (Cell Signaling #9662 Danvers, MA, USA), GAPDH 1:2,000 (Cell Signaling #2118), β-tubulin 1:1,500 (Cell Signaling #2146), phospho-4E-BP1 1:1,000 (S65, Cell Signaling #9451), 4E-BP1 1:1,000 (Cell Signaling #9452), phospho-S6K1 1:1,000 (T389, Cell Signaling #9205), c-Myc 1:5,000 (Abcam #32072), S6K1 1:1,000 (Cell Signaling #2708), phospho-AKT 1:1,000 (S473, Cell Signaling #9271), AKT 1:1,000 (Cell Signaling #9272), phospho-EGFR 1:1,000 (Y1068, Cell Signaling #3777), EGFR 1:1,000 (Cell Signaling #4267) ANO1 1:500 (Abcam ab64085), phospho-CAMKIIα 1:1,000 (T286, Cell Signaling #12716) and CAMKII 1:1,000 (Cell Signaling #11945). Uncropped immunoblot images are included in Supplementary Fig. 8 .
Phospho camkiiα
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-CAMKIIα is a primary antibody that recognizes the phosphorylated form of Calcium/calmodulin-dependent protein kinase II alpha. It can be used to detect the activation state of this important signaling enzyme.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Cell Signaling Technology (2 mentions)
Phospho s6k1, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Camkii, by Cell Signaling Technology (1 mentions)
Phospho egfr, by Cell Signaling Technology (1 mentions)
Hif 1α, by Cayman Chemical (1 mentions)
Phospho 4ebp1, by Cell Signaling Technology (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Hif 2α, by Novus Biologicals (1 mentions)
C myc, by Abcam (1 mentions)
Psd95, by Cell Signaling Technology (1 mentions)
Camkiiα, by Cell Signaling Technology (1 mentions)
Phospho pka, by Cell Signaling Technology (1 mentions)
K48 polyubiquitin, by Cell Signaling Technology (1 mentions)
2 protocols using phospho camkiiα
1
Western Blot Analysis of Protein Signaling
2
Protein Signaling Pathway Evaluation
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Rabbit primary antibodies used were as follows: K48 polyubiquitin (1:1000; Cell Signaling Technology 8081), phospho-Rpt6 (Ser120; 1:1000; Signalway Antibody 12880), Rpt6 (1:1000; Cell Signaling Technology 13392), phospho-PKA (Thr197; 1:1000; Cell Signaling Technology 5661), PKA (1:1000; Cell Signaling Technology 4782), phospho-CaMKIIα (Thr286; 1:1000; Cell Signaling Technology 12716), CaMKIIα (1:1000; Cell Signaling Technology 4436), PSD95 (1:1000; Cell Signaling Technology 2507), Arc/Arg3.1 (1:1000; Cell Signaling Technology 65650), and GAPDH (1:1000; Cell Signaling Technology 2118).
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