Sureprint g3 human cgh microarray 8 x 60 k kit

Array CGH analysis was performed using a SurePrint G3 Human CGH Microarray 8 X 60 K kit (Agilent Technologies, Santa Clara, CA, USA), which consisted of 62,976 oligonucleotide probes spaced at 41 kbp intervals (median probe spacing) throughout the genome. Control DNA (Promega Corp., Nepean, Canada) was used as the reference DNA. DNA digestion, labeling and hybridization were performed following the manufacturer’s instructions. Scanned images were quantified using Agilent Feature Extraction software (v10.0), and the resulting data were imported into Agilent Genomic Workbench 7.0.4.0 software for visualization, and copy number variations were detected using the Aberration Detection Method-2 (ADM-2) algorithm. All genomic coordinates were based on human genome build hg19/GRCh37.

Genomic DNA was extracted from peripheral blood using the QIAamp DNA Mini Kit (Qiagen). The DNA was quantified spectrophotometrically using a ND‐1000 (Nanodrop Technologies). Array‐based comparative genomic hybridization analysis was then performed with a SurePrint G3 Human CGH Microarray 8x60K kit (Agilent Technologies), which consisted of 62,976 oligonucleotide probes spaced at 41 kbp intervals (median probe spacing) throughout the genome. Normal male or female DNA (Agilent Technologies) was used as controls. DNA digestion, labeling, and hybridization were performed according to the manufacturers' instructions. Scanned images were quantified using Agilent Feature Extraction software (v10.0), and the resulting data were imported into Agilent Genomic Workbench 7.0.4.0 software for visualization. Copy number variations were detected, using the Aberration Detection Method‐2 (ADM‐2) algorithm. Genomic positions were defined according to the GRCh37/hg19 Assembly of the Human Genome (February 2009).