After 7 days in culture, EPCs from db/+ mice were reseeded into six-well plates at 2 × 105 cells/well and incubated at 37°C until the cells were 60%–80% confluent. After that, EPCs were serum starved for 6 h before small interfering (si)RNA transfection. The EPC-conditioned media on CSE-siRNA (sc-142618, Santa Cruz Biotechnology) was diluted to a 10 μmol/L working solution and delivered to cells at a 5 nmol/L final concentration through a siRNA transfection reagent (sc-29528, Santa Cruz Biotechnology). After 5–7 h of transfection, the transfection mixture was removed and replaced with fresh EGM-2 and incubated for an additional 48 h. A nonrelated scrambled sequence siRNA (Control siRNA-A, sc-37007; Santa Cruz Biotechnology) was used as a transfection control.
Sc 142618
Manufactured by Santa Cruz Biotechnology
Sc-142618 is a laboratory reagent developed by Santa Cruz Biotechnology. It is a purified protein that can be used as a tool in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Control sirna a sc 37007, by Santa Cruz Biotechnology (1 mentions)
Sc 29528, by Santa Cruz Biotechnology (1 mentions)
Cse sirna, by Santa Cruz Biotechnology (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Sc 36869, by Santa Cruz Biotechnology (1 mentions)
Raw264.7 cells, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
2 protocols using sc 142618
1
Silencing CSE in Mouse Endothelial Progenitor Cells
2
Silencing CSE in RAW264.7 Macrophages
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The murine macrophage cell line, RAW264.7 cells (American Type Culture Collection), were cultured and propagated in DMEM medium supplemented with 10% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin (GIBCO) at 37°C in humidified atmosphere of 5% CO 2 .
siRNA interference Mouse RAW264.7 macrophages were transfected with mouse CSE-specific siRNA (sc-142618) or control siRNA (sc-36869) (both from Santa Cruz Biotechnology, Santa Cruz, CA) using Lipofectamine RNAiMAX (Life Technologies, Shanghai, China) according to the manufacturer's instructions. Briefly, individual siRNAs (at 25 to 50 nM), Lipofectamine RNAiMAX, and Opti-MEM were mixed and incubated at room temperature for 5 min. siRNA-Lipofectamine RNAiMAX complexes were added to cells for 24 h and the medium was replaced by fresh serum DMEM medium after transfection. Experiments were performed 72 h after transfection. Knockdown of CSE was assessed by Western blot.
siRNA interference Mouse RAW264.7 macrophages were transfected with mouse CSE-specific siRNA (sc-142618) or control siRNA (sc-36869) (both from Santa Cruz Biotechnology, Santa Cruz, CA) using Lipofectamine RNAiMAX (Life Technologies, Shanghai, China) according to the manufacturer's instructions. Briefly, individual siRNAs (at 25 to 50 nM), Lipofectamine RNAiMAX, and Opti-MEM were mixed and incubated at room temperature for 5 min. siRNA-Lipofectamine RNAiMAX complexes were added to cells for 24 h and the medium was replaced by fresh serum DMEM medium after transfection. Experiments were performed 72 h after transfection. Knockdown of CSE was assessed by Western blot.
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