To knockdown Notch1, lentivirus vectors which expressed mouse Notch1 shRNA and scrambled shRNA were obtained from Sigma (Sigma Co., Ltd., Ronkonkoma, NY, USA). The Notch1 shRNA lentivirus was obtained as previously described [30 (link)]. The scrambled shRNA sequence (5′-TTCTCCGAACGTGTCACGT-3′) or Notch1 shRNA sequence (5′-CCGGCCCACATTCCAGAGGCATTTACTCGAGTAAATGCCTCTGGAATGTGGGTTTTT-3′) was subcloned into the pLV/H1-GFP plasmid. Recombinant lentivirus titer was adjusted to 1 × 109 TU/mL.
Lentivirus (3 mL) was stereotaxically injected into the right lateral ventricle (0.7 mm outside to the midline, 0.1 mm posterior to bregma, and 2.2 mm deep). All implanted mice were operated as sham or MCAO model at day 14 after virus injection.
Lentivirus (3 mL) was stereotaxically injected into the right lateral ventricle (0.7 mm outside to the midline, 0.1 mm posterior to bregma, and 2.2 mm deep). All implanted mice were operated as sham or MCAO model at day 14 after virus injection.