Hek293t

Huh7, HEK 293T cells stably expressing TLR3 (generous gifts from Dr. Kate Fitzgerald), HEK 293T (Dharmacon, Inc.), and Vero (obtained from American Type Culture Collection (ATCC)) cells were maintained in Dulbecco’s modified minimal essential medium and HepG2 (ATCC) cells were maintained in Eagle’s Minimum Essential Medium, both supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 1% penicillin-streptomycin (Sigma-Aldrich), 1% non-essential amino acids (Lonza) and 1% L-Glutamine solution (Sigma-Aldrich). U937 cells stably expressing DC-SIGN (a generous gift from Dr. Anuja Mathew) were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 1% penicillin-streptomycin (Sigma-Aldrich), 1% non-essential amino acids (Lonza), and 1% L-Glutamine solution (Sigma-Aldrich). All cells were incubated in a humidified chamber at 37°C and 5% CO2. DENV2 strains DENV2 16681 and NGC were originally obtained from ATCC or the Walter Reed Army Institute of Research and were passaged in C6/36 cells (ATCC). Virus titers were determined by immunostained plaque assay on Vero cells (Liu et al., 2012 (link); Medin et al., 2015 (link)).

Human primary lung embryonic fibroblasts MRC5 (PD 5-40, ATCC, USA) and Virus-packaging GP293 cells (Clontech, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum and penicillin/streptomycin 100 U ml^-1. Human primary prostate fibroblasts (PD 0-15, ScienCell, USA) were cultured in MCDB131 medium (Biological Industries, Israel) supplemented with 10% foetal bovine serum, 10 mM Hepes, 1 ng ml⁻¹ bFGF and 0.1 ng ml⁻¹ EGF. CAFs from human prostate cancer biopsies were obtained by explant as described in our previous paper^{61 (link)}.
PC3, PC3-Luc and DU145 epithelial prostate cancer cells (ATCC) were cultured in RPMI 1640 supplemented with 10% FBS and 1% l-glutamine. HEK293T (Dharmacon) and GP293 packaging cells were cultured in DMEM containing 10% FBS. The cells were maintained at 37 °C under a 10% CO₂ atmosphere.
Sf-9 insect cells (Novagen) were cultured in Grace’s Insect Medium, supplemented with 5% FBS at 28 °C in a ventilated incubator. All the media and supplements, if not stated otherwise, were from Gibco (CA, USA).