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Neurofax

Manufactured by Nihon Kohden
Sourced in Japan

The Neurofax is a diagnostic device designed to record and analyze electrical activity in the brain. It serves as a comprehensive solution for conducting electroencephalography (EEG) tests, providing healthcare professionals with valuable insights into the brain's functioning.

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10 protocols using neurofax

1

EEG Sleep Analysis for Neurosurgical Patients

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EEG was recorded for the analysis before the surgery using the international 10–20 electrode system (Neurofax; Nihon‐Kohden, Tokyo, Japan). The sampling rate was 500 Hz. A low‐cut frequency (LCF) filter at 0.016 Hz was used before digital sampling. We initially reviewed sleep EEG using a bipolar montage, including the derivations of Fp1‐F7, Fp2‐F8, F3‐C3, F4‐C4, T3‐T5, T4‐T6, P3‐O1, and P4‐O2 to select clean data.
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2

EEG Recording and Analysis Protocol

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Subsequent routine EEG recordings were obtained using an 18-channel digital EEG machine (Neurofax; Nihon-Kohden, Tokyo, Japan) with electrode placement according to the International EEG 10–20 system, as described previously [4 (link),11 (link),12 ,16 (link)]. Evaluation of the EEG findings was based on visual inspection by two board certified electroencephalographers (T.M. and A.S.) who were blinded to the clinical data. No differences in the electroencephalographers' interpretations were noted on independent assessments (kappa = 1) [7 ]. As previously described [11 (link)], three kinds of EEG findings, namely, repeated ictal discharges, repetitive epileptic discharges, and periodic lateralized epileptic discharges were included in the ongoing ictal EEG findings.
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3

Polysomnographic Sleep Monitoring Protocol

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Polysomnography was performed using a digital (computerized) system (Neurofax, NIHON KOHDEN, Inc., Tokyo, Japan). It simultaneously recorded multiple physiological variables related to sleep staging: an electroencephalogram (EEG), eye movement (electrooculogram: EOG), chin muscle activity (by EMG), respiration (nasal and oral airflow), breathing effort (measured at the chest and abdomen), oximetry, heart rate, electrocardiogram (ECG), sound of snoring, body position, and the recording of leg anterior tibialis muscle activity (by EMG). Airflow was measured by thermocouples and by nasal cannula pressure. Nasal prongs were used to measure the nasal pressure signal.
The exploring electrode of the EEG was placed over specific anatomical sites: C3, C4, O1, and O2 (C3, C4, O1, and O2 are symbols of the international 10/20 system of scalp electrode placement identifying the central electrode placement site over the left [C3, O1] and right [C4, O2] hemispheres of the brain.). The time scale used in the polysomnography was 10 mm/s.
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4

Extracranial EEG Electrode Placement Protocol

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For extracranial EEG recording, disc electrodes were placed over the scalp according to the international 10–20 EEG system. Moreover, T1/T2 electrodes[11 (link)] and periorbital electrodes (supraorbital lateral: SOL, infraorbital lateral: IOL, and infraorbital medial: IOM)[10 ] were placed [Figure 1a]. The MA electrodes were placed on the MA angles on both sides and the CH electrode was placed on the CH at the midline of the mandible [Figures 1a and b]. CH and MA electrodes are farther from the mesial temporal lobe compared to other extracranial electrodes [Figures 1a and b]. He underwent simultaneous recordings of extra- and intracranial EEG as part of a presurgical evaluation, as described previously.[2 (link),7 (link)-9 (link)] EEG recordings were collected using a digital EEG recorder (Neurofax: Nihon Kohden, Tokyo, Japan).
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5

Overnight Polysomnography Assessment

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Overnight PSG was performed the night before the clinical assessments and followed a night of sleep adaptation to the PSG with one exception; that participant completed the PSG 2 nights before the clinical assessments and without an adaptation night. EEGs were recorded using a Nihon-Kohden system (Neurofax version 05–50; Nihon-Kohden) and Polysmith Acquisition and Review Software (version 4.0.25.0; Nihon-Kohden). Electrooculograms and submental electromyograms were used to evaluate eye movements and muscle activity, respectively. All EEGs were scored by reviewers blinded to diagnosis using guidelines established by Rechtschaffen and Kales (24 ). Further description of the summary PSG scores and power spectral density estimates can be found in the Supplemental Methods.
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6

Scalp Video-EEG Acquisition and Electrode Placement

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Scalp video-EEGs were performed using NicoletOne or BMSI6000 (Natus Medical Incorporated, WI) for patients 1–4, 6–9, and 11–16, and Neurofax (Nihon-Kohden, Japan) for patients 5, 10, and 17. EEG was sampled at 256 Hz (patients 3, 8, 12, and 16), 400 Hz (patients 1, 2, 4, 7, 11, 14, and 15), 500 Hz (patients 5, 10, and 17), 512 Hz (patients 6 and 13), and 1,024 Hz (patient 9). Electrodes were placed according to the international 10/20 system, using at least 16 EEG channels (Fp1, Fp2, F3, F4, C3, Cz, C4, P3, P4, O1, O2, F7, F8, T3, T4, T5, and T6). The ground electrode was set attached to the frontal pole (Fpz). Electromyogram (EMG) electrodes were placed on both deltoid muscles.
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7

Preoperative and Postoperative EEG in CHS

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Twenty-nine of the 32 patients underwent routine EEG recording preoperatively and on POD1. Follow-up EEG was performed in 3 patients who developed CHS. Routine EEG recordings were obtained from an 18-channel digital EEG machine (Neurofax; Nihon-Kohden, Tokyo, Japan) with electrode placement according to the International EEG 10-20 system. The EEG recordings were performed for at least 30 min for each patient at rest.
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8

Scalp EEG Characterization in Neurocritical Care

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Digital scalp EEG (Neurofax; Nihon-Kohden, Tokyo, Japan) was recorded with the international 10–20 system electrode placement and additional T1/T2 electrodes. The EEG recordings were performed for at least 1 h for all patients. Continuous EEG monitoring (more than 6 h) was performed in 15 patients. PDs and RDA were identified using standardized critical care EEG terminology of the American Clinical Neurophysiology Society (Hirsch et al., 2013 (link)). The PDs were subcategorized as lateralized, bilateral independent and generalized PDs. The RDA was subcategorized as lateralized, bilateral independent and generalized RDA. The EEG seizures were identified as frequency change and apparent spatiotemporal evolution pattern of epileptiform discharges with a frequency of >25 times per 10 s epoch. Brief rhythmic epileptiform discharges were identified as repetitive epileptiform discharges occurring two or three times per second, lasting only a few seconds. Single spike or sharp waves were not evaluated in this study. Two experienced, board-certified experts of EEG and clinical neurophysiology (S.O., H.O.) independently reviewed the recordings of EEG, unaware of the patients’ clinical data including ASL.
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9

Electrophysiological Recordings and Electrode Localization

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Electrophysiological methods and electrode localization were similar to those described previously39 (link),40 (link). In brief, subdural circular platinum-iridium electrodes with a top hat design (4.5-mm overall diameter, 3-mm cortical contact, 10-mm interelectrode distance) were implanted and placed solely on the basis of clinical considerations using standard techniques41 . Electrode localization was verified by co-registering a post-operative CT imaging with a pre-operative MRI structural image. Lobar and gyral labels were assigned by an expert in human neuroanatomy (N.T.). ECoG signals were sampled at 1000 Hz using Nihon Kohden NeuroFax (Japan) with a recording bandwidth from 0.15 to 300 Hz. Signals were referenced to a common average consisting of all non-ictal electrodes over lateral frontal and lateral temporal areas to minimize the effect of the referencing scheme on synchronization measures42 . Recordings were then imported into MATLAB for post-processing. All the data used in the analysis originated from recordings free from seizure events.
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10

EEG Synchrony and Asymmetry Analysis

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EEG was recorded using the standard international 10–20 system, with a sampling frequency of 500 Hz (Neurofax; Nihon-Kohden, Tokyo, Japan). A low-cut filter at 1.6 Hz was used before digital sampling. A 4-h VEEG monitoring was performed. All cases must complete 4-h surveillance, including at least one complete wake-sleep cycle. All EEGs were analyzed by one EEG technician and at least two certified epileptologists in order to reach consensus conclusions. According to Andre M, et al.14 (link), asynchrony implied a temporal delay longer than 1.5 to 2 s between the bursts of identical waveforms on both hemispheres, and the amplitude difference exceeding 50% over one hemisphere was considered as an abnormal EEG. We, therefore, defined interhemispheric synchrony as that a temporal delay was no longer than 1.5 to 2 s between the bursts of identical waveforms between hemispheres, and bilateral hemispheric asymmetry as that the amplitude of BS pattern in one hemisphere was 50% higher than the other.
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