Gentamycin

Manufactured by Teknova

Gentamycin is a laboratory equipment product used as an antibiotic agent. It is a broad-spectrum aminoglycoside antibiotic effective against a variety of gram-negative and some gram-positive bacteria.

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Lab products found in correlation

4 protocols using gentamycin

Isolation of Primary Human Tissue Cells

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Adult human primary tissue samples were obtained from National Disease Research Interchange (NDRI). Fetal and embryonic tissue samples were obtained from Novogenix Laboratories. All donated material was anonymous, carried no personal identifiers and was obtained after informed consent. Sex of the specimens was unknown. Human primary tissues, hES-derived chondrocytes on membranes, or hBMSCs on membranes were manually cut into small pieces and digested 4–16 h at 37 °C with mild agitation in digestion media consisting of DMEM/F12 (Corning) with 10% FBS (Corning), 1 mg/mL dispase (Gibco), 1 mg/mL type 2 collagenase (Worthington), 10 µg/mL gentamycin (Teknova) and 100 μg/ml primocin (Invivogen).

Petrigliano F.A., Liu N.Q., Lee S., Tassey J., Sarkar A., Lin Y., Li L., Yu Y., Geng D., Zhang J., Shkhyan R., Bogdanov J., Van Handel B., Ferguson G.B., Lee Y., Hinderer S., Tseng K.C., Kavanaugh A., Crump J.G., Pyle A.D., Schenke-Layland K., Billi F., Wang L., Lieberman J., Hurtig M, & Evseenko D. (2021). Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. NPJ Regenerative Medicine, 6, 77.

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Isolation of Primary Tissue Samples

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Adult human primary tissue samples were obtained from National Disease Research Interchange (NDRI). Fetal and embryonic tissue samples were obtained from Novogenix Laboratories. All donated material was anonymous, carried no personal identifiers and was obtained after informed consent. Sex of the specimens was unknown. Pig cartilage and synovium were harvested from pig legs supplied by S&S Farms. Human and pig primary tissues were manually cut into small pieces and digested 4–16 h at 37 °C with mild agitation in digestion media consisting of DMEM (Corning) with 10% FBS (Sigma), 1 mg/mL dispase (Gibco), 1 mg/mL type 2 collagenase (Worthington), 10 –µg/mL gentamycin (Teknova) and primocin (Invivogen). Mouse cartilage was digested for 3–4 h and then sorted.

Ferguson G.B., Van Handel B., Bay M., Fiziev P., Org T., Lee S., Shkhyan R., Banks N.W., Scheinberg M., Wu L., Saitta B., Elphingstone J., Larson A.N., Riester S.M., Pyle A.D., Bernthal N.M., Mikkola H.K., Ernst J., van Wijnen A.J., Bonaguidi M, & Evseenko D. (2018). Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nature Communications, 9, 3634.

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Metabolic Pathway Analysis with Isotopic Labeling

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The following reagents were obtained from Sigma-Aldrich: glucose, 1,3-propanediol, phenylboronic acid, cycloheximide, 23BD, acetoin, pyruvate, PEP, 6PG, G6P, F6P, Ru5P, R15P, ATP, dithiothreitol (DTT), NADH, NADPH, NADP⁺, phosphocreatine, RuBisCO, pyruvate kinase, lactate dehydrogenase, G6P dehydrogenase, 3PGA kinase, GAPDH and creatine kinase. U-¹³C glucose and ¹³C-NaHCO₃ were obtained from Cambridge Isotope Laboratories. IPTG and chloramphenicol were obtained from Fischer Scientific. Gentamycin was purchased from Teknova. Spectinomycin was purchased from MP Biomedicals. Kanamycin was purchased from IBI Scientific. Phusion polymerase was purchased from New England Biolabs. All oligonucleotide synthesis and DNA sequencing were performed by Eurofins MWG Operon Inc.

Kanno M., Carroll A.L, & Atsumi S. (2017). Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria. Nature Communications, 8, 14724.

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Murine Cartilage Cell Isolation

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Mouse knee joints were cut at the femur and the tibia and crushed lightly with a mortar and pestle and placed in digestion media (DMEM/F12 (Corning) with 10% Fetal Bovine Serum (FBS; Corning), 1% penicillin/streptomycin/amphotericin B solution (P/S/A; Corning), 1 mg/ml dispase (Gibco), 1 mg/ml type 2 collagenase (Worthington), 10 µg/ml gentamycin (Teknova) and 100 μg/ml primocin (Invivogen) in an Erlenmeyer flask with a spin bar in 4 °C overnight followed by 37 °C for 4–6 h. Cells were washed with DPBS (Corning) after digestion and strained through a 70 μm filter (Fisher) before FACS.

Liu N.Q., Lin Y., Li L., Lu J., Geng D., Zhang J., Jashashvili T., Buser Z., Magallanes J., Tassey J., Shkhyan R., Sarkar A., Lopez N., Lee S., Lee Y., Wang L., Petrigliano F.A., Van Handel B., Lyons K, & Evseenko D. (2022). gp130/STAT3 signaling is required for homeostatic proliferation and anabolism in postnatal growth plate and articular chondrocytes. Communications Biology, 5, 64.

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